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Purification of the EGF Receptor for Oligosaccharide Studies

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Glycoprotein Analysis in Biomedicine

Part of the book series: Methods in Molecular Biology ((MIMB,volume 14))

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Abstract

The epidermal growth factor receptor is a membrane glycoprotein expressed in a large variety of higher eukaryotic cells. Its role in growth and development as well as its high levels of expression in certain types of cancer have stimulated wide-ranging studies at the tissue, cell, and molecular levels. The receptor is a 175-kDa single polypeptide protein consisting of an extracellular EGF binding domain, a transmembrane peptide, and an intracellular protein tyrosine kinase domain (1,2). The extracellular domain contains 12 potential glycosylation sites and is heavily glycosylated (3–5). The structures of some of these carbohydrate antigens have been identified by either determining the specificity of monoclonal antibodies raised against the receptor or, conversely, by investigating the reactivity of unrelated monoclonals of known antigenic specificity with purified receptor (6,7).

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Author information

Authors and Affiliations

  1. Laboratory of Molecular Biophysics, University of Oxford, UK

    Mary Gregoriou

Authors
  1. Mary Gregoriou
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Editor information

Editors and Affiliations

  1. Medical Research Council, Clinical Research Centre, Harrow, UK

    Elizabeth F. Hounsell

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© 1993 Humana Press Inc., Totowa, NJ

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Gregoriou, M. (1993). Purification of the EGF Receptor for Oligosaccharide Studies. In: Hounsell, E.F. (eds) Glycoprotein Analysis in Biomedicine. Methods in Molecular Biology, vol 14. Humana Press. https://doi.org/10.1385/0-89603-226-4:189

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  • DOI: https://doi.org/10.1385/0-89603-226-4:189

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-226-2

  • Online ISBN: 978-1-59259-501-3

  • eBook Packages: Springer Protocols

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