Skip to main content

Environmental Influences on Cells in Culture

  • Protocol
Practical Cell Culture Techniques

Part of the book series: Neuromethods ((NM,volume 23))

Abstract

The technique of culturing cells derived from the nervous system has been in use for over 80 years, with the objective of simplifying the experimental system to provide readily manipulatable models of neural function. These preparations are useful for testing hypotheses relevant to cell adhesion, motility, survival, proliferation, longevity, and expression of cell type-specific properties. Many significant modifications of these methods have resulted over the years, as new data have emerged from cell biology and neurobiology studies (see reviews by Bottenstein, 1983a,Bottenstein, 1985,Bottenstein, 1988).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Bottenstein J. (1983a) Growth requirements of neural cells in vitro. Adv. Cell. Neurobiol. 4, 333–379.

    CAS  Google Scholar 

  2. Bottenstein J. (1983b) Defined media for dissociated neural cultures, in Current Methods in Cellular Neurobiology (Barker J. and McKelvy J, eds.), John Wiley, New York, pp. 107–130.

    Google Scholar 

  3. Bottenstein J. (1984) Culture methods for growth of neuronal cell lines in defined media, in Methods in Molecular and Cell Biology, vol. 4: Methods for Serum-free Culture of Neuronal and Lymphoid Cells (Barnes D., Sirbasku D., and Sato G., eds.), Alan Liss, New York, pp. 3–13.

    Google Scholar 

  4. Bottenstein J. (1985) Growth and differentiation of neural cells in defined media, in Cell Culture in the Neurosciences (Bottenstein J. and Sato G., eds.), Plenum, New York, pp. 3–43.

    Chapter  Google Scholar 

  5. Bottenstein J. (1986) Growth requirements in vitro of oligodendrocyte cell lines and neonatal rat brain oligodendrocytes. Proc. Natl. Acad. Sci. USA 83, 1955–1959.

    Article  PubMed  CAS  Google Scholar 

  6. Bottenstein J. (1988) Advances in vertebrate cell culture methods. Science 239, G42,G48.

    Article  PubMed  CAS  Google Scholar 

  7. Bottenstein J. and Hunter S. (1990) Culture methods for oligodendrocyte cell lines and oligodendrocyte-type 2 astrocyte lineage cells, in Methods in Neurosciences, vol. 2: Cell Culture (Conn P. M., ed.), Academic, San Diego, pp. 56–75.

    Google Scholar 

  8. Bottenstein J. and Michler-Stuke A. (1983) Proliferation of glial-derived cell lines in serum-free defined medium, in Developing and Regenerating Vertebrate Nervous Systems, vol 6: Neurology and Neurobiology (Coates P., Markwald R., and Kenny A., eds.), Alan Liss, New York, pp. 185–189.

    Google Scholar 

  9. Bottenstein J. and Michler-Stuke A. (1989) Serum-free culture of dissociated neonatal rat cortical astrocytes, in A Dissection and Tissue Culture Manual of the Nervous System (Shahar A., de Vellis J., Vernadakis A., and Haber B., eds.), Alan Liss, New York, pp. 109–111.

    Google Scholar 

  10. Bottenstein J. and Sato G. (1979) Growth of a rat neuroblastoma cell line in serum-free supplemented medium. Proc. Natl. Acad. Sci. USA 76, 514–517.

    Article  PubMed  CAS  Google Scholar 

  11. Bottenstein J. and Sato G. (1980) Fibronectin and polylysine requirement for proliferation of neuroblastoma cells in defined medium. Exp. Cell Res. 129, 361–366.

    Article  PubMed  CAS  Google Scholar 

  12. Bottenstein J., Hunter S., and Seidel M. (1988) CNS neuronal cell line-derived factors regulate gliogenesis in neonatal rat brain cultures. J. Neurosci. Res. 20, 291–303.

    Article  PubMed  CAS  Google Scholar 

  13. Bottenstein J., Skaper S., Varon S., and Sato G. (1980) Selective survival of neurons from chick embryo sensory ganglionic dissociates utilizing serum-free supplemented medium. Exp. Cell Res. 125, 183–190.

    Article  PubMed  CAS  Google Scholar 

  14. Hughes S.M., Lillien L., Raff M., Rohrer H., and Sendtner M. (1988) Ciliary neurotrophic factor induces type-2 astrocyte differentiation in culture. Nature 335, 70–72.

    Article  PubMed  CAS  Google Scholar 

  15. Hunter S. and Bottenstein J. (1989) Bipotential glial progenitors are targets of neuronal cell line-derived growth factors. Develop. Brain Res. 49, 33–49.

    Article  CAS  Google Scholar 

  16. Hunter S. and Bottenstein J. (1990) Growth factor responses of enriched bipotential glial progenitors. Develop. Brain Res. 54, 235–248.

    Article  CAS  Google Scholar 

  17. Hunter S. and Bottenstein J. (1991) O-2A glial progenitors from mature brain respond to CNS neuronal cell line-derived growth factors. J. Neurosci. Res. 28, 574–582.

    Article  PubMed  CAS  Google Scholar 

  18. Kingsbury A., Gallo V., Woodhams P., and Balazs R. (1985) Survival, morphology and adhesion properties of cerebellar interneurons cultured in chemically defined and serum-supplemented medium. Develop. Brain Res. 17, 17–25.

    Article  Google Scholar 

  19. Marangos P. and Schmechel D. (1987) Neuron specific enolase, a clinically useful marker for neurons and neuroendocrine cells. Ann. Rev. Neurosci. 10, 269–295.

    Article  PubMed  CAS  Google Scholar 

  20. Michler-Stuke A. and Bottenstein, J. (1982a) Proliferation of glial-derived cells in defined media. J. Neurosci. Res. 7, 215–228.

    Article  PubMed  CAS  Google Scholar 

  21. Michler-Stuke A. and Bottenstein J. (1982b) Defined media for growth of human and rat glial-derived cell lines, in Cold Spring Harbor Conferences on Cell Proliferation, vol. 9 (Sirbasku D., Sato G., and Pardee A., eds.), Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp. 959–971.

    Google Scholar 

  22. Michler-Stuke A., Wolff J.R., and Bottenstein, J. (1984) Factors influencing astrocyte growth and development in defined media. Int. J. Develop. Neurosci. 2, 575–584.

    Article  Google Scholar 

  23. Miller R. and Raff M. (1984) Fibrous and protoplasmic astrocytes are biochemically and developmentally distinct. J. Neurosci. 4, 585–592.

    PubMed  CAS  Google Scholar 

  24. Raff M., Miller R.H., and Noble, M. (1983) A glial progenitor cell that develops in vitro into an astrocyte or an oligodendrocyte depending on culture medium. Nature 303, 390–396.

    Article  PubMed  CAS  Google Scholar 

  25. Raff M., Abney E.R., and Miller R.H. (1984) Two glial cell lineages diverge prenatally in rat optic nerve. Develop. Biol. 106, 53–60.

    Article  PubMed  CAS  Google Scholar 

  26. Wilkin G., Levi G., Johnstone S., and Riddle P. (1983) Cerebellar astroglial cells in primary culture: expression of different morphological appearances and different ability to take up [3H]D-aspartate and [3H]GABA. Develop. Brain Res. 10, 265–277.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1992 The Humana Press Inc. Totowa, New Jersey

About this protocol

Cite this protocol

Bottenstein, J.E. (1992). Environmental Influences on Cells in Culture. In: Boulton, A.A., Baker, G.B., Walz, W. (eds) Practical Cell Culture Techniques. Neuromethods, vol 23. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-214-0:63

Download citation

  • DOI: https://doi.org/10.1385/0-89603-214-0:63

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-214-9

  • Online ISBN: 978-1-59259-628-7

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics