Skip to main content
SpringerLink
Log in

Brain Capillaries

Cell Culture of Capillary Endothelium Derived from Cerebral Microvessels

  • Protocol
Practical Cell Culture Techniques

Part of the book series: Neuromethods ((NM,volume 23))

Abstract

The microvasculature of the brain is important in the normal development and maintenance of the blood-brain barrier. Since glial cells are in close contact with brain capillaries in situ, communication between glial cells and capillary endothelial cells is thought to be important in maintaining homeostasis within the central nervous system. The selective permeability barrier, termed the blood-brain barrier, is maintained by specific membrane transport systems and tight junctions between adjacent endothelial cells. (Patho)physiological stresses such as anoxia, ischemia, metabolic, and/or degenerative disturbances, affect the endothelium, and in turn, affect the selective permeability barrier. Brain capillary endothelial cells in culture would facilitate the study of the mechanisms involved in cerebral vascular injury.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Alles J. U. and Bosslet K. (1986) Immunohistochemical and immunochemical characterization of a new endothelial cell specific antigen. Histochem. Cytochem. 34, 209–214.

    Article  CAS  Google Scholar 

  2. Berger C. N. (1986) In situ hybridization of immunoglobin-specific RNA in single cells of the B lymphocyte lineage with radiolabelled DNA probes. EMBO J. 5, 85–93.

    PubMed  CAS  Google Scholar 

  3. Berman L., McLeod M. P., and Powsner E. P. (1965) Monitor cultures of stored frozen human cells. Lab. Invest. 14, 231–234.

    PubMed  CAS  Google Scholar 

  4. Bowman P. D., Betz A. L., Ar D., Wolinsky J. S., Penney J. B., Shivers R. R., and Goldstein G. W. (1981) Primary cultures of capillary endothelium from rat brain. In Vitro 17, 353–362.

    Article  PubMed  CAS  Google Scholar 

  5. Bruzney S. M. and Massicotte S. J. (1979) Retinal Vessels: Proliferation of endothelium in vitro. Invest. Opth. Vis. Sci. 18, 1191–1195.

    Google Scholar 

  6. Carrel A. and Burrows M. T. (1910) Cultivation of adult tissues and organs outside of the body. J. Am. Med. Assoc. 55, 1379–1381.

    Article  Google Scholar 

  7. Davidson P. M., Bensch K., and Karasek M. A. (1980) Isolation and growth of endothelial cells from the microvessels of the newborn human foreskin in cell culture. J. Invest. Derm. 75, 316–321.

    Article  Google Scholar 

  8. Davison P. M. and Karasek M. A. (1981) Human dermal microvascular endothelial cells in vivo: effect of cyclic AMP on cellular morphology and proliferation rate. J. Cell Physiol. 106, 253–258.

    Article  PubMed  CAS  Google Scholar 

  9. De Bault L. E., Henriquez E., Hart M. N., and Cancilla P. A. (1981) Cerebral microvessels and derived cells in tissue culture: II Establishment, identification and preliminary characterization of an endothelial cell line. In Vitro 17, 480–494.

    Article  Google Scholar 

  10. De Bault L. E., Kahn L. E., Frommes S. P., and Cancilla P. A. (1979) Cerebral microvessels and derived cells in tissue culture: isolation and primary characterization. In Vitro 15, 473–487.

    Article  Google Scholar 

  11. De Bault L. E. and Cancilla P. A. (1980) λ-glutamyl transpeptidase in isolated brain endothelial cell: Induction by glial cells in vitro. Scence 207, 653–655.

    Article  Google Scholar 

  12. Del Vecchio P. J., Ryan V. S., and Ryan J. W. (1977) Isolation of capillary segments from rat adrenal gland. J. Cell Biol. 75, 73a.

    Google Scholar 

  13. Feig C. (1910) Sur la survir d’elements et de systemes cellulaires, en particulair des vaisseaux apres conservation prolongie hors de l’organisme. C.R. Soc. Biol. 69, 504–509.

    Google Scholar 

  14. Fishman J. B., Andraham J. V., Connor J., Dickey B. F., and Fine R. T. (1985) Receptor-mediated transcytosis of transferrin across the blood brain barrier. J. Cell Biol. 101, 423a.

    Google Scholar 

  15. Folkman J. and Haudenschild C. C. (1980) Angiogenesis in vitro. Nature 288, 551–556.

    Article  PubMed  CAS  Google Scholar 

  16. Folkman J., Haudenschild C. C., and Zetter B. R. (1979) Long term culture of capillary endothelial cells. Proc. Natl. Acad. Sri. USA 76, 5217–5221.

    Article  CAS  Google Scholar 

  17. Frank R. N., Kinsey V. E., Frank K. W., Mickus K. P., and Randolph A. (1979) In vitro proliferation of endothelial cells from kitten retinal capillaries. Invest. Opth. Vis. Sci. 18, 1195–1200.

    CAS  Google Scholar 

  18. Freshney R. I., ed. (1983) Culture of Animal Cell: A Journal of Basic Techniques. Alan R. Liss, New York.

    Google Scholar 

  19. Greene A. E., Athreya B. H., Lehr H. B., and Coriell L. L. (1967) Viability of cell cultures following extended preservation in liquid nitrogen. Proc. Soc. Exp. Biol. Med. 124, 1302–1307.

    Article  PubMed  CAS  Google Scholar 

  20. Greene A. E., Manduka M., and Coriell L. L. (1975) Viability of cell cultures stored up to 12 years in liquid nitrogen. Cryobiology 12, 583.

    Article  Google Scholar 

  21. Hayes L. W., Goguen C. A., Ching S. F., and Slakey L. L. (1978) Angiotensin converting enzyme: Accumulation in medium from cultured endothelial cells. Biochem. Biophys. Res. Commun. 82, 1147–1153.

    Article  PubMed  CAS  Google Scholar 

  22. Jakoby W. B. and Pastan I. H., eds. (1979) Cell culture. Methods in Enzymology vol. LVIII. Academic, New York, NY.

    Google Scholar 

  23. Jefferies W. A., Brandon M. R., Hunt S. V., Williams A. F., Gatter K. C., and Mason D. Y. (1984) Transferrin receptor on endothelium of brain capillaries. Nature. 312, 162–163.

    Article  PubMed  CAS  Google Scholar 

  24. Johnson A. R. and Erdos E. G. (1977) Metabolism of vasoactive peptides by human endothelial cells in culture: Angiotensin I converting enzyme and angiotensinase. J. Clin. Invest. 59, 684–695.

    Article  PubMed  CAS  Google Scholar 

  25. Johnson A. R. (1980) Human pulmonary endothelial cells in culture: Activities of cells from arteries and cells from veins. J. Clin. Invest. 65, 841–850.

    Article  PubMed  CAS  Google Scholar 

  26. Karasek M. and Charlton M. (1974) Isolation and growth of skin endothelial cells in cell culture. J. Invest. Derm. 62, 542.

    Google Scholar 

  27. Ketis N. V., Hoover R. L., and Karnovsky M. J. (1988a) Effects of hyperthermia on cell survival and patterns of protein synthesis in endothelial cells from different origins. Cancer Res. 48, 2101–2106.

    PubMed  CAS  Google Scholar 

  28. Ketis N. V., Lawler J., Hoover R. L., and Karnovsky M. J. (1988b) Effects of heat shock on the expression of thrombospondin by endothelial cells in culture. J. Cell Biol. 106, 893–904.

    Article  PubMed  CAS  Google Scholar 

  29. Kruse, Jr., P. F. and Patterson, Jr., M. K., eds. (1973) Tissue Culture Methods and Applications. Academic, New York, NY.

    Google Scholar 

  30. McGarrity G. J. (1975) Tissue Cult. Assoc. 1, 181–192.

    Article  Google Scholar 

  31. Mrsulja B. B., Mrsulja B. J., Fujimoto T., Klatzo I., and Spatz M. (1976) Isolation of brain capillaries a simple technique. Brain Res. 110, 361–365.

    Article  PubMed  CAS  Google Scholar 

  32. Olesen S. P. and Crone C. (1984) Serotonin increases microvascular premeability in the brain. Int. J. Microcirc. Clin. Exp. 3, 466–471.

    Google Scholar 

  33. Palade G. E., Simionescu M., and Simionescu N. (1979) Structural aspects of the permeability of the microvascular endothelium. Acta Physiol. Scand. Suppl. 463, 11–32.

    PubMed  CAS  Google Scholar 

  34. Panula P., Joo F., and Rechardt L. (1978) Evidence for the presence of viable endothelial cells in cultures derived from dissociated rat brain. Experimentia 34, 95–97.

    Article  CAS  Google Scholar 

  35. Paul J. (1975) Cell and Tissue Culture, 5th ed., Churchill-Livingston, London.

    Google Scholar 

  36. Penson G. and Balducci D., eds., (1963) Tissue cultures in biological research. Elsevier, Amsterdam.

    Google Scholar 

  37. Peterson W. P. and Stulberg C. (1965) Cytobiology 1, 80–92.

    Google Scholar 

  38. Phillips P., Kumar P., Kumar S., and Waghe M. (1979) Isolation and characterization of endothelial cells from rat and cow brain white matter. J. Anat. 129, 261–272.

    PubMed  CAS  Google Scholar 

  39. Simionescu M., Simionescu N., and Palade G. E. (1975) Segmental differentiations of cell junctions in vascular endoethelium. The Micovasculature. J. Biol. Chem. 67, 863–869.

    CAS  Google Scholar 

  40. Simionescu M., Simionescu N., and Palade G. E. (1976) Segmental differentiations of cell junctions in the vascular endothelium. Arteries and veins. J. Cell Biol. 68, 705–723.

    Article  PubMed  CAS  Google Scholar 

  41. Spatz M., Bembry J., Dodson R. F., Hervonen H., and Murray M. R. (1980) Endothelial cell culture derived from isolated cerebral microvessels. Brain Res. 191, 577–582.

    Article  PubMed  CAS  Google Scholar 

  42. Strieter R. M., Kunkel S. L., and Showell H. J. (1989) Endothelial cell gene expression of a neutrophil chemotactic factor by TNF-α, LPS and IL-13. Science 243, 1467–1469.

    Article  PubMed  CAS  Google Scholar 

  43. Taub M. and Sato G. (1980) Growth of functional primary cultures of kidney epithelial cells in defined medium. J. Cell. Physiol. 105, 369–378.

    Article  PubMed  CAS  Google Scholar 

  44. Wagner R. C. and Matthews M. A. (1975) The isolation and culture of capillary endothelium from epididymal fat. Microvasc. Res. 10, 286–297.

    Article  PubMed  CAS  Google Scholar 

  45. Williams S. K., Gillis J. F., Matthews M. A., Wagner R. C. and Bitensky M. W. (1980) Isolation and characterization of brain endothelial cells: Morphology and Enzyme Activity. J. Neurochem. 35, 374–381.

    Article  PubMed  CAS  Google Scholar 

  46. Zetter B. R., Johnson L. K., Shuman M. A., and Gospodarowicz D. (1978) The isolation of vascular endothelial cell lines with altered cell surface and platelet-binding properties. Cell 14, 501–509.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Anatomy, Queen’s University, Kingston, Ontario, Canada

    Nika V. Ketis

Authors
  1. Nika V. Ketis
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. University of Saskatchewan, Saskatoon, Canada

    Alan A. Boulton

  2. University of Alberta, Edmonton, Canada

    Glen B. Baker

  3. University of Saskatchewan, Saskatoon, Canada

    Wolfgang Walz

Rights and permissions

Reprints and permissions

Copyright information

© 1992 The Humana Press Inc. Totowa, New Jersey

About this protocol

Cite this protocol

Ketis, N.V. (1992). Brain Capillaries. In: Boulton, A.A., Baker, G.B., Walz, W. (eds) Practical Cell Culture Techniques. Neuromethods, vol 23. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-214-0:353

Download citation

  • DOI: https://doi.org/10.1385/0-89603-214-0:353

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-214-9

  • Online ISBN: 978-1-59259-628-7

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation