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Practical Protein Chromatography pp 223–236Cite as

Size-Exclusion High-Performance Liquid Chromatography of Proteins

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 11))

Abstract

In chromatography, a solute partitions between a mobile and a stationary phase. Uniquely in size-exclusion chromatography, both phases have identical physicochemical properties; differential partition is effected by restricting the access of the solute to the stationary phase. At its simplest, the stationary phase is held inside pores with a distribution of sizes, and some pores are too small to permit the entry of larger molecules—hence size exclulsion (SE) (or the alternative gel permeation) chromatography.

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Author information

Authors and Affiliations

  1. Department of Biochemistry, Oxford University, South Parks Road, Oxford, UK

    David A. Harris

Authors
  1. David A. Harris
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Editor information

Editors and Affiliations

  1. Drew Scientific Limited, London

    Andrew Kenney

  2. Leicester Polytechnic, Scraptoft, Leicester, UK

    Susan Fowell

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© 1992 Humana Press Inc., Totowa, NJ

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Harris, D.A. (1992). Size-Exclusion High-Performance Liquid Chromatography of Proteins. In: Kenney, A., Fowell, S. (eds) Practical Protein Chromatography. Methods in Molecular Biology™, vol 11. Humana Press. https://doi.org/10.1385/0-89603-213-2:223

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  • DOI: https://doi.org/10.1385/0-89603-213-2:223

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-213-2

  • Online ISBN: 978-1-59259-498-6

  • eBook Packages: Springer Protocols

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