Size-Exclusion High-Performance Liquid Chromatography of Proteins
In chromatography, a solute partitions between a mobile and a stationary phase. Uniquely in size-exclusion chromatography, both phases have identical physicochemical properties; differential partition is effected by restricting the access of the solute to the stationary phase. At its simplest, the stationary phase is held inside pores with a distribution of sizes, and some pores are too small to permit the entry of larger molecules—hence size exclulsion (SE) (or the alternative gel permeation) chromatography.
KeywordsHPLC DMSO Agarose Helium Electrophoresis
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