Size-Exclusion High-Performance Liquid Chromatography of Proteins

  • David A. Harris
Part of the Methods in Molecular Biology™ book series (MIMB, volume 11)

Abstract

In chromatography, a solute partitions between a mobile and a stationary phase. Uniquely in size-exclusion chromatography, both phases have identical physicochemical properties; differential partition is effected by restricting the access of the solute to the stationary phase. At its simplest, the stationary phase is held inside pores with a distribution of sizes, and some pores are too small to permit the entry of larger molecules—hence size exclulsion (SE) (or the alternative gel permeation) chromatography.

Keywords

HPLC DMSO Agarose Helium Electrophoresis 

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Copyright information

© Humana Press Inc., Totowa, NJ 1992

Authors and Affiliations

  • David A. Harris
    • 1
  1. 1.Department of BiochemistryOxford UniversityOxfordUK

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