Abstract
The genetic disorders of hemoglobin, notably, sickle cell anemia and the α- and β-thalassemia, are the commonest genetic diseases in humans. Furthermore, the majority of these mutant globin genes, particularly those causing β-thalassemia, are owing to point mutations that do not involve cleavage sites for restriction enzyme, which means that allele-specific oligonucleotide probe hybridization has become indispensable for the direct detection of these point mutations.
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References
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© 1991 The Humana Press Inc., Clifton, NJ
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Thein, S.L. (1991). The Detection of Point Mutations in Hemoglobin Defects Using Allele-Specific Oligonucleotide Probes. In: Mathew, C.G. (eds) Protocols in Human Molecular Genetics. Methods in Molecular Biology, vol 9. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-205-1:287
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DOI: https://doi.org/10.1385/0-89603-205-1:287
Publisher Name: Springer, Totowa, NJ
Print ISBN: 978-0-89603-205-7
Online ISBN: 978-1-59259-496-2
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