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Gene Targeting for Somatic Cell Manipulation

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Protocols in Human Molecular Genetics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 9))

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Abstract

Recent technological advances, not least in somatic cell genetics, have accelerated progress in human genome mapping and reverse genetics. In particular, chromosome mediated gene transfer (CMGT) (1) and irradiation and fusion gene transfer (IFGT) (2) have been exploited to great effect for enrichment cloning of predetermined regions of the human genome. Both methods allow subchromosomal fragments of human DNA (generally 1 to 50 Mbp in size) to be isolated onto a rodent cell background as a resource for DNA cloning and fine structure mapping.

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Author information

Authors and Affiliations

  1. MRC Human Genetics Unit, Western General Hospital, Edinburgh, UK

    Julia R. Dorin & David J. Porteous

Authors
  1. Julia R. Dorin
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  2. David J. Porteous
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Editor information

Editors and Affiliations

  1. United Medical and Dental Schools of Guy’s, St. Thomas’s Hospitals, London, UK

    Christopher G. Mathew

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© 1991 The Humana Press Inc., Clifton, NJ

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Dorin, J.R., Porteous, D.J. (1991). Gene Targeting for Somatic Cell Manipulation. In: Mathew, C.G. (eds) Protocols in Human Molecular Genetics. Methods in Molecular Biology, vol 9. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-205-1:223

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  • DOI: https://doi.org/10.1385/0-89603-205-1:223

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-205-7

  • Online ISBN: 978-1-59259-496-2

  • eBook Packages: Springer Protocols

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