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Yeast Artificial-Chromosome (YAC) Cloning Systems

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Protocols in Human Molecular Genetics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 9))

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Abstract

Yeast artificial-chromosome (YAC) cloning systems are used to clone large contiguous segments of DNA from any organism into suitable vectors in such a way that the recombinants can be transformed into yeast (Saccharomyces cerevisiae) cells, where they are stably propagated. The basic strategy for producing artificial chromosomes in yeast was first described in 1983 by Murray and Szostak (1). In 1987, Burke et al. (2) demonstrated that artificial chromosomes in yeast were useful for cloning very large segments of exogenous DNA.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Genetics, St. Mary’s Hospital Medical School, London, UK

    Michele Ramsay & Carol Wicking

Authors
  1. Michele Ramsay
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  2. Carol Wicking
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Editor information

Editors and Affiliations

  1. United Medical and Dental Schools of Guy’s, St. Thomas’s Hospitals, London, UK

    Christopher G. Mathew

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© 1991 The Humana Press Inc., Clifton, NJ

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Ramsay, M., Wicking, C. (1991). Yeast Artificial-Chromosome (YAC) Cloning Systems. In: Mathew, C.G. (eds) Protocols in Human Molecular Genetics. Methods in Molecular Biology, vol 9. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-205-1:197

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  • DOI: https://doi.org/10.1385/0-89603-205-1:197

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-205-7

  • Online ISBN: 978-1-59259-496-2

  • eBook Packages: Springer Protocols

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