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Gene Targeting for Somatic Cell Manipulation

  • Julia R. Dorin
  • David J. Porteous
Part of the Methods in Molecular Biology book series (MIMB, volume 9)

Abstract

Recent technological advances, not least in somatic cell genetics, have accelerated progress in human genome mapping and reverse genetics. In particular, chromosome mediated gene transfer (CMGT) (1) and irradiation and fusion gene transfer (IFGT) (2) have been exploited to great effect for enrichment cloning of predetermined regions of the human genome. Both methods allow subchromosomal fragments of human DNA (generally 1 to 50 Mbp in size) to be isolated onto a rodent cell background as a resource for DNA cloning and fine structure mapping.

Keywords

Hypoxanthine Phosphoribosyl Transferase Homologous Recombination Event Somatic Cell Genetic Polymerase Chain Reaction Screening Homologous Recombination Frequency 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© The Humana Press Inc., Clifton, NJ 1991

Authors and Affiliations

  • Julia R. Dorin
    • 1
  • David J. Porteous
    • 1
  1. 1.MRC Human Genetics UnitWestern General HospitalEdinburgh

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