Discontinuous Polyacrylamide Gel Electrophoresis of DNA Fragments

  • Bruce Budowle
  • Robert C. Allen
Part of the Methods in Molecular Biology book series (MIMB, volume 9)

Abstract

At present, the most polymorphic genetic markers are DNA regions composed of a variable number of tandem repeats (VNTRs) (1,2). The detection of VNTRs is made possible by restriction fragment-length polymorphism (RFLP) analysis via Southern blotting (3). The length of each DNA fragment is a function of the number of tandem repeats contained within it. The RFLP technique requires at least 10–50 ng of essentially undegraded DNA (10,000–20,000 bp) and isotopic detection to obtain a result; the time required for analysis may be in excess of one week (4).

Keywords

Formalin Glycerol Agarose Electrophoresis Fractionation 

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Copyright information

© The Humana Press Inc., Clifton, NJ 1991

Authors and Affiliations

  • Bruce Budowle
    • 1
  • Robert C. Allen
    • 2
  1. 1.Forensic Science Research and Training CenterLaboratory Division, FBI AcademyQuantico
  2. 2.Department of PathologyMedical University of South CarolinaCharleston

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