Discontinuous Polyacrylamide Gel Electrophoresis of DNA Fragments

  • Bruce Budowle
  • Robert C. Allen
Part of the Methods in Molecular Biology book series (MIMB, volume 9)


At present, the most polymorphic genetic markers are DNA regions composed of a variable number of tandem repeats (VNTRs) (1,2). The detection of VNTRs is made possible by restriction fragment-length polymorphism (RFLP) analysis via Southern blotting (3). The length of each DNA fragment is a function of the number of tandem repeats contained within it. The RFLP technique requires at least 10–50 ng of essentially undegraded DNA (10,000–20,000 bp) and isotopic detection to obtain a result; the time required for analysis may be in excess of one week (4).


Ammonium Persulfate Silver Nitrate Solution Lead Sulfate Agarose Plug VNTR Locus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© The Humana Press Inc., Clifton, NJ 1991

Authors and Affiliations

  • Bruce Budowle
    • 1
  • Robert C. Allen
    • 2
  1. 1.Forensic Science Research and Training CenterLaboratory Division, FBI AcademyQuantico
  2. 2.Department of PathologyMedical University of South CarolinaCharleston

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