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Nonisotopic In Situ Hybridization

Immunocytochemical Detection of Specific Repetitive Sequences on Chromosomes and Interphase Nuclei

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Immunochemical Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 10))

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Abstract

The term in situ hybridization describes a wide range of techniques concerned with the detection of DNA or RNA sequences within individual cells, tissues, or on identifiable regions of chromosomes. The technique utilizes an ability to label DNA or RNA probes so that, following hybridization with complementary sequences in the target tissues, the labeled DNA or RNA can be detected by various techniques.

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Author information

Authors and Affiliations

  1. Wessex Regional Genetics Laboratory, General Hospital, Salisbury, UK

    John A. Crolla

Authors
  1. John A. Crolla
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Editor information

Editors and Affiliations

  1. Medical Research Council Laboratories, Carshalton, Surrey, UK

    Margaret M. Manson

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© 1992 The Humana Press, Inc., Totowa, NJ

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Crolla, J.A. (1992). Nonisotopic In Situ Hybridization. In: Manson, M.M. (eds) Immunochemical Protocols. Methods in Molecular Biology, vol 10. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-204-3:431

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  • DOI: https://doi.org/10.1385/0-89603-204-3:431

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-204-0

  • Online ISBN: 978-1-59259-497-9

  • eBook Packages: Springer Protocols

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