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Colloidal Gold Staining and Immunodetection in 2-D Protein Mapping

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Immunochemical Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 10))

Abstract

This chapter extends the use of the technique described in the previous chapter to two-dimensional (2D) protein gels, as well as containing some alternatives and modifications to the method.

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References

  1. O’Farrell, P. H. (1975) High resolution two dimensional electrophoresis of proteins. J. Biol. Chem. 250, 4007–4021.

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  2. O’Farrell, P. Z., Goodman, H. M., and O’Farrell, P. H. (1977) High resolution two dimensional electrophoresis of basic as well as acidic proteins. Cell, 12, 1133–1142.

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  3. Righetti, P. G., Casero, P., and Del Campo, G. B. (1986) Gold staining in cellulose acetate membranes. Clin. Chem. Acta. 157, 167–174.

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  4. Schapira, A. H. V. and Keir, G. (1988) Two dimensional protein mapping by gold stain and immunoblotting. Analyt. Biochem. 169, 167–171.

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Author information

Authors and Affiliations

  1. Department of Neurological Science, Royal Free Medical School, London, UK

    Anthony H. V. Schapira

  2. Institute of Neurology, London, UK

    Anthony H. V. Schapira

Authors
  1. Anthony H. V. Schapira
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Editor information

Editors and Affiliations

  1. Medical Research Council Laboratories, Carshalton, Surrey, UK

    Margaret M. Manson

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© 1992 The Humana Press, Inc., Totowa, NJ

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Schapira, A.H.V. (1992). Colloidal Gold Staining and Immunodetection in 2-D Protein Mapping. In: Manson, M.M. (eds) Immunochemical Protocols. Methods in Molecular Biology, vol 10. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-204-3:255

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  • DOI: https://doi.org/10.1385/0-89603-204-3:255

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-204-0

  • Online ISBN: 978-1-59259-497-9

  • eBook Packages: Springer Protocols

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