Flow Cytometric Analysis of Cells Using an Antibody to a Surface Antigen

  • Michael G. Ormerod
Part of the Methods in Molecular Biology book series (MIMB, volume 10)


Identifying a subset of cells, particularly peripheral blood lymphocytes, by means of a fluorescently-tagged antibody is one of the commonest applications of flow cytometry. There are many methods, all of them basically similar, for labeling antigens on cell surfaces. Two slightly different procedures are given in this chapter; these can be adapted to meet most needs.


Propidium Iodide Propidium Iodide Flow Cytometric Analysis Peripheral Blood Lymphocyte Light Scatter 
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  1. 1.
    Ormerod, M. G., ed. (1990) Flow Cytometry: A Practical Approach. Oxford University Press, Oxford.Google Scholar
  2. 2.
    Carter, N. P. (1990) Measurement of cellular subsets using antibodies, in Flow Cytometry: A Practical Approac h (Ormerod, M. G., ed.) Oxford University Press, Oxford, pp. 45–67.Google Scholar

Copyright information

© The Humana Press, Inc., Totowa, NJ 1992

Authors and Affiliations

  • Michael G. Ormerod
    • 1
  1. 1.Institute of Cancer ResearchThe Haddow LaboratoriesSuttonUK

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