Flow Cytometric Analysis of Cells Using an Antibody to a Surface Antigen
Identifying a subset of cells, particularly peripheral blood lymphocytes, by means of a fluorescently-tagged antibody is one of the commonest applications of flow cytometry. There are many methods, all of them basically similar, for labeling antigens on cell surfaces. Two slightly different procedures are given in this chapter; these can be adapted to meet most needs.
KeywordsPropidium Iodide Propidium Iodide Flow Cytometric Analysis Peripheral Blood Lymphocyte Light Scatter
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