Avidin–Biotin Technology

Preparation of Avidin Conjugates
  • Edward A. Bayer
  • Meir Wilchek
Part of the Methods in Molecular Biology book series (MIMB, volume 10)


The second step required in the application of avidin-biotin technology is to prepare an appropriate avidin-associated probe or probes for the desired application (for general reviews, see refs. 1 and 2). For example, a fluorescent form of avidin can be used for fluorescence microscopy, fluorescence-activated cell sorting, and in some cases, for immunoassay. Like-wise, an avidin-enzyme conjugate can be used for immunoblotting, immunoassay, light microscopy, and in some cases, electron microscopy. An immobilized form of avidin can be used for isolation purposes (see Note 1).


Sodium Azide Sodium Borohydride Void Volume Fraction Sodium Bicarbonate Solution Sodium Periodate 
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  1. 1.
    Wilchek, M. and Bayer, E. A., eds. (1990) Avidin-Biotin Technology. Methods in Enzymology, vol. 184, Academic, Orlando, FL.Google Scholar
  2. 2.
    Wilchek, M. and Bayer, E. A. (1988) The avidin-biotin complex in bioanalytical applications. Anal. Biochem. 171, 1–32.PubMedCrossRefGoogle Scholar

Copyright information

© The Humana Press, Inc., Totowa, NJ 1992

Authors and Affiliations

  • Edward A. Bayer
    • 1
  • Meir Wilchek
    • 1
  1. 1.Department of BiophysicsThe Weizmann Institute of ScienceRehovotIsrael

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