Abstract
All cells have a large number of proteins; for instance, a single hepatocyte has about lo4 different proteins, and a number of them are involved in tissue-specific functions. In neuronal cells, there are additional 104 protein species, which are predicted, from kinetic of DNA-RNA hybridization studies, to be involved in specialized neural functions and in intricate systems of communication (1,2). At present, it is possible to separate and analyze numerous proteins using two-dimensional gel electrophoresis; however, only around 103 protein species in a given tissue can be detected by this method even when combined with the silver-staining technique. In contrast, if a cDNA library from mRNAs is screened, using molecular biological techniques, about 105–106 independent clones, each of which is expected to encode different proteins, can be easily isolated and screened. Recent advances in the techniques of genetic engineering encourage the neuroscientist to search for molecules underlying neuronal functions.
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© 1992 The Humana Press, Totowa, NJ
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Kuo, CH. (1992). Isolation of Photoreceptor Cell-Specific MEKA cDNA by Differential Hybridization. In: Longstaff, A., Revest, P. (eds) Protocols in Molecular Neurobiology. Methods in Molecular Biology™, vol 13. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-199-3:79
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DOI: https://doi.org/10.1385/0-89603-199-3:79
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