Abstract
Recent progress in the field of molecular biology has provided a new method to localize an mRNA signal within a cell or tissue section. This technique, called “in situ hybridization histochemistry,” gives us dynamic information about gene expression at individual cellular lev- els. For the detection of a particular target mRNA, a specific probe that has complementary sequence to the target mRNA is used. This probe has to include a reporter molecules in order to visualize the position of the probe-mRNA hybrid on a tissue section (1). Conven- tional in situ hybridization procedures use radioisotopes (e.g., 3H, 35S, 32P) as reporter molecules that can be visualized by autoradiography after hybridization (2–4). The use of autoradiography, both film auto- radiography and emulsion autoradiography, restricts the wide appli- cation of this method because of the following reasons.
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© 1992 The Humana Press, Totowa, NJ
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Kiyama, H., Emson, P.C., Tohyama, M. (1992). In Situ Hybridization Histochemistry Using Alkaline Phosphatase-Labeled Oligodeoxynucleotide Probe. In: Longstaff, A., Revest, P. (eds) Protocols in Molecular Neurobiology. Methods in Molecular Biology™, vol 13. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-199-3:167
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DOI: https://doi.org/10.1385/0-89603-199-3:167
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