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Design and Construction of Poliovirus Epitope Expression Vectors

  • Protocol
Practical Molecular Virology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 8))

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Abstract

We have developed the very safe and efficacious live-attenuated Sabin 1 poliovirus vaccine strain as a vehicle for the presentation of defined epitopes from foreign pathogens (1–3). Precise modification of the poliovirus capsid is made possible by the application of recombinant DNA technology to cloned cDNA copies of the viral genome, which are infectious for mammalian cells in culture (4).

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References

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Author information

Authors and Affiliations

  1. Institut für Mikrobiologie der Universitat Ulm, Abteilung Virologie, Ulm, Federal Republic of Germany

    Karen L. Burke

  2. Department of Microbiology, University of Reading, Reading, UK

    Jeffrey W. Almond & David J. Evans

Authors
  1. Karen L. Burke
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  2. Jeffrey W. Almond
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  3. David J. Evans
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Editor information

Editors and Affiliations

  1. Chester Beatty Laboratories, Institute of Cancer Research, London, UK

    Mary K. L. Collins

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© 1991 The Humana Press Inc., Clifton, NJ

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Burke, K.L., Almond, J.W., Evans, D.J. (1991). Design and Construction of Poliovirus Epitope Expression Vectors. In: Collins, M.K.L. (eds) Practical Molecular Virology. Methods in Molecular Biology, vol 8. Humana Press. https://doi.org/10.1385/0-89603-191-8:249

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  • DOI: https://doi.org/10.1385/0-89603-191-8:249

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-191-3

  • Online ISBN: 978-1-59259-495-5

  • eBook Packages: Springer Protocols

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