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Calcium Phosphate Mediated Gene Transfer into Established Cell Lines

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Gene Transfer and Expression Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 7))

Abstract

DNA transfection is one of the most important techniques in molecular genetics. It is this technique that has made possible the dissection of complex eukaryotic genes and the characterization of the function of their components (17) as well as the isolation of particular genes on the basis of their expression in cultured cells (811).

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© 1991 The Humana Press Inc., Clifton, NJ

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Okayama, H., Chen, C. (1991). Calcium Phosphate Mediated Gene Transfer into Established Cell Lines. In: Murray, E.J. (eds) Gene Transfer and Expression Protocols. Methods in Molecular Biology, vol 7. Humana Press. https://doi.org/10.1385/0-89603-178-0:15

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  • DOI: https://doi.org/10.1385/0-89603-178-0:15

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-178-4

  • Online ISBN: 978-1-59259-494-8

  • eBook Packages: Springer Protocols

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