Chromosome-Mediated Gene Thnsfer

  • Janet M. Shipley
  • Denise Sheer
Part of the Methods in Molecular Biology book series (MIMB, volume 7)

Abstract

McBride and Ozer (1) were the first to show that purified metaphase chromosomes could act as vectors in transferring genetic information into mammalian cells. This technique, termed chromosome-mediated gene transfer (CMGT), involves the transfer of subchromosomal fragments from the cells of one species (the donor, usually human) to those of another (the recipient, usually a rodent) that have the ability to maintain them. CMGT thus fills a gap between the isolation of whole chromosomes, produced by cell fusion and microcell-mediated gene transfer, and the isolation of relatively short stretches of DNA by cloning or transfection methods.

Keywords

Glycerol DMSO Leukemia Recombination Electrophoresis 

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Copyright information

© The Humana Press Inc., Clifton, NJ 1991

Authors and Affiliations

  • Janet M. Shipley
    • 1
  • Denise Sheer
    • 1
  1. 1.Human Cytogenics LaboratoryImperial Cancer Research FundLondonUK

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