Chromosome-Mediated Gene Thnsfer

  • Janet M. Shipley
  • Denise Sheer
Part of the Methods in Molecular Biology book series (MIMB, volume 7)


McBride and Ozer (1) were the first to show that purified metaphase chromosomes could act as vectors in transferring genetic information into mammalian cells. This technique, termed chromosome-mediated gene transfer (CMGT), involves the transfer of subchromosomal fragments from the cells of one species (the donor, usually human) to those of another (the recipient, usually a rodent) that have the ability to maintain them. CMGT thus fills a gap between the isolation of whole chromosomes, produced by cell fusion and microcell-mediated gene transfer, and the isolation of relatively short stretches of DNA by cloning or transfection methods.


Thymidine Kinase Recipient Cell Somatic Cell Hybrid Selective Strategy Dominant Selectable Marker 
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  1. 1.
    McBride, O. W. and Ozer, H. L. (1973) Transfer of genetic information by purified metaphase chromosomes. Prot. Natl. Acad. Sci. USA 70, 1258–1262.CrossRefGoogle Scholar
  2. 2.
    Weiss, J. H., Nelson, D. L., Pryborski, M. J., Chaplin, D. D., Mulligan R. C., Housman, D. E., and Seidman, J. G. (1984) Eukaryotic chromosome transfer. Linkage of the murine major histocompatability complex to an inserted selectable marker. Proc. Natl. Acad. Sci. USA 81. 4879–4883.CrossRefGoogle Scholar
  3. 3.
    Spandidos, D. A. and Siminovitch, L. (1977) Transfer of codominant markers by isolated metaphase chromosomes in Chinese hamster ovary cells.Proc. Natl. Acud Sci. USA 74, 3480–3484.CrossRefGoogle Scholar
  4. 4.
    Gross, T. A., Squires, S., Martin, P., and Baker, and R. M. (1979) Chromosome-mediated gene transfer in an mtraspecific human cell system. J. Cell Biol. 83, 4Google Scholar
  5. 5.
    Porteous, D. J., Morten J. E. N., Cranston, G., Fletcher, J. M., Mitchell, A., van Heyningen, V., Fantes,J. A., Boyd, P. A., and Hastie, N. D. (1986) Molecular and physical arrangements of human DNA in HRAS-1 selected chromosome mediated transfectants. Mol. Cell. Biol. 6, 2223–2232.PubMedGoogle Scholar
  6. 6.
    Morten, J. E. N., Hirst, M. C., and Porteous, D. J. (1987) The c-H-ras-1 oncogene in chromosome mediated gene transfer. [itAnlicancer Res. , 573–588.Google Scholar
  7. 7.
    Scambler, P. J., Law, H. Y, Williamson, R., and Cooper, C. S. (1987) Chromosome mediated gene transfer of six DNA markers linked to the cystic fibrosis locus on human chromosome seven. Nuckic Acid Res. 14, 7159–7174.CrossRefGoogle Scholar
  8. 8.
    van Heyningen, V. and Porteous, D. J. (1986) Mapping a chromosome to find a gene. Trends Genet. 2, 4,5.Google Scholar
  9. 9.
    Murphy, P. D., Miller, C.L., and Ruddle, F.H. (1985) Qrantitahon of the transgenome size in chromosome-mediated gene transfer lines. Cytogenet. Cell Genet. 39, 125–133.PubMedCrossRefGoogle Scholar
  10. 10.
    Klobutcher, L. A. and Ruddle, F. H. (1979) Phenotype stabilization and integration of transferred material in chromosome-mediated gene transfer. [itNature [bd280, 657–660.Google Scholar
  11. 11.
    Xu, W., Gorman, P. A, Rider, S. H., Hedge, P. J., Moore, G, Pritchard, C., Sheer, D., and Solomon, E. (1988) Construction of a genetic map of the human chromosome 17 by use of chromosome mediated gene transfer. Proc. Natl. Acad. Sci. USA 85, 8563–8567.CrossRefGoogle Scholar
  12. 12.
    Murphy, P. D. and Ruddle, F. H. (1985) Isolation and regional mapping of random X sequences from distal human X chromosome. somat. [itCell Mol. Genet. 11, 433–444.Google Scholar
  13. 13.
    Goodfellow, P. N., Banting, G., Wiles, M. V., Tunnacliffe, A., Parkar, M., Solomon, E., Dalchau, R., and Fabre, J. W. (1982) The gene, MIC4, which controls expression of the antigen defined by monoclonal antibody F1 0.44.2, is on human chromosome 11. Eur.J. Immunol. 12, 659–663.PubMedCrossRefGoogle Scholar
  14. 14.
    Kuhn, L. C., McClelland, A., and Ruddle, F. H. (1984) Gene transfer, expression, and molecular cloning of the human transferrin gene. cell 37, 95–103.PubMedCrossRefGoogle Scholar
  15. 15.
    McAlpine, P. J., Shows, T. B., Miller, R. L., Boucheix, C., Stranc, L. C., Berent, T. G., Pakstis, A.J., and Douté, R. C. (1989) The 1989 catalog of mapped genes and report of the nomenclature committee. Cyfogenet. Cell Genet. 51, 13–66.CrossRefGoogle Scholar
  16. 16.
    Porteous, D. J. (1987) Chromosome mediated gene transfer: A functional assay for complex loci and an aid to human genome mapping. Trends tenet. 3, 171–182.Google Scholar
  17. 17.
    Pritchard, C. and Goodfellow, P. (1986) Development of new methods in human genome mapping: Selection for fragments of the human Ychromosome after chromosome mediated gene transfer. EMBOJ. 5, 979–985.Google Scholar
  18. 18.
    Pritchard, C. and Goodfellow, P. N. (1987) Inveshgation of chromosome-mediated gene transfer using the HPRT region of the human X chromosome as a model. Genes Deu. 1, 172–178.CrossRefGoogle Scholar
  19. 19.
    Siden, T. S., Hoglund, M., and Rohme, D. (1989) Construction of microcell hybrid panel containing different neo gene insertions in mouse chromosome 17 used for chromosome mediated gene transfer. Somat. [itCell Mol Genet. 15, 245.Google Scholar
  20. 20.
    Capecchi, M. R. (1989) The new mouse genetics: Altering genome by gene targeting. Trends Genet. 5, 70–76.PubMedCrossRefGoogle Scholar
  21. 21.
    Dorin, J. R., Inglis, J. D., and Porteous, D. J. (1989) Selection for precise chromosomal targeting of a dominant marker by homologous recombination. science 243, 1357–1360.PubMedCrossRefGoogle Scholar
  22. 22.
    Goodfellow, P. N. and Pritchard, C. A. (1988) Chromosome fragmentation by chromosome mediated gene transfer. Cancer Surv. 7, 25l–265.Google Scholar
  23. 23.
    Brickmore, W. A., Maule, J. C., van Heyningen, V., and Porteous, D. J. (1989) Longrange structure of H-ras l-selected transgenomes. Somat. CellMol. Cenet. 15, 229–235.Google Scholar
  24. 24.
    Gusella, J. F., Jones, C., Kao, F., Housman, D., and Puck, T. T. (1982) Genetic finestructure in human chromosome 11 by use of repetitive DNA sequences. Proc. Natl. Acad. Sci. USA 79, 7804–7808.PubMedCrossRefGoogle Scholar
  25. 25.
    Goss, S. J. and Harris, H. (1975) New method for mapping genes in human chromosomes. Nature 255, 680–683.PubMedCrossRefGoogle Scholar
  26. 26.
    Benham, F., Hart, K., Crolla, J., Bobrow, M., Francavilla, M., and Goodfellow, P. N. (1989) A method for generating hybrids containing nonselected fragments of human chromosomes. Genomics 4, 509–517.PubMedCrossRefGoogle Scholar

Copyright information

© The Humana Press Inc., Clifton, NJ 1991

Authors and Affiliations

  • Janet M. Shipley
    • 1
  • Denise Sheer
    • 1
  1. 1.Human Cytogenics LaboratoryImperial Cancer Research FundLondonUK

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