Preparation of Recombinant Plasmid DNA for DNA-Mediated Gene Transfer

  • Rémy Aubin
  • Michael Weinfeld
  • Malcolm C. Paterson
Part of the Methods in Molecular Biology book series (MIMB, volume 7)

Abstract

Recombinant plasmid constructs are frequently employed in transfection experiments. With the availability of a wide spectrum of specialized and versatile eucaryotic cloning/expression vectors, investigators have been given powerful tools to expedite the elucidation of mechanisms governing gene expression, and to facilitate the identification of genes participating in diverse cellular processes (namely, metabolism, the immune response, differentiation and development, the repair of DNA damage, and malignant transformation).

Keywords

Vortex Filtration Phenol Chloroform Syringe 

References

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Copyright information

© The Humana Press Inc., Clifton, NJ 1991

Authors and Affiliations

  • Rémy Aubin
    • 1
  • Michael Weinfeld
    • 1
  • Malcolm C. Paterson
    • 1
  1. 1.Molecular Genetics and Carcinogenesis LaboratoryW. W. Cross Cancer InstituteEdmontonCanada

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