Abstract
Plant tissues grown in vitro provide an ideal research tool for the study of a wide range of aspects of plant science. For example, they have been used in the investigation of both primary and secondary metabolism, cytodifferentiation, morphogenesis, plant tumor physiology, and the formation of plant hybrids via protoplast fusion techniques. Plant tissue culture is also being increasingly adopted for the commercial propagation of plants.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bioas-says with tobacco tissue cultures. Physiol Plant 15,473–497.
White, P. R. (1954) The Cultivation of Animal and Plant Cells. (The Ronald Press, New York).
Gambourg, O. L., Miller, R. A., and Ojima, K. (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50,151–158.
Brown, J. T. and Charlwood, B. V. (1986) The control of callus formation and differentiation in scented Pelargoniums. J. Plant Physiol. 123,409–417.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Humana Press Inc.
About this protocol
Cite this protocol
Brown, J.T. (1990). The Initiation and Maintenance of Callus Cultures. In: Pollard, J.W., Walker, J.M. (eds) Plant Cell and Tissue Culture. Methods in Molecular Biology™, vol 6. Humana Press. https://doi.org/10.1385/0-89603-161-6:57
Download citation
DOI: https://doi.org/10.1385/0-89603-161-6:57
Publisher Name: Humana Press
Print ISBN: 978-0-89603-161-6
Online ISBN: 978-1-59259-493-1
eBook Packages: Springer Protocols