Abstract
Various immobilization strategies have been developed to optimize the biosynthetic potential of cultured plant cells. Immobilization involves the retaining of suspension-cultured plant cells on, or within, a physical barrier that promotes cell aggregation and separates the cells from the surrounding media. The advantages of immobilization include the formation of diffusionary gradients around and between the cells that increase intercellular biochemical communication conducive to the coordinated expression of secondary metabolism. In effect, immobilization simulates the physiological conditions within large aggregates of cells in a manner amenable to manipulation for fermentation purposes.
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References
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© 1990 Humana Press Inc.
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Facchini, P.J., DiCosmo, F., Radvanyi, L.G., Neumann, A.W. (1990). Immobilization of Cells by Spontaneous Adhesio. In: Pollard, J.W., Walker, J.M. (eds) Plant Cell and Tissue Culture. Methods in Molecular Biology™, vol 6. Humana Press. https://doi.org/10.1385/0-89603-161-6:513
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DOI: https://doi.org/10.1385/0-89603-161-6:513
Publisher Name: Humana Press
Print ISBN: 978-0-89603-161-6
Online ISBN: 978-1-59259-493-1
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