Abstract
The chloroplast genome encodes a number of proteins, including thylakoid proteins and the large subunit of ribulose biphosphate carboxylase, associated with the structure and function of the chloroplast (1-2). In addition, many components of the chloroplast translational machinery, such as all of the RNAs and some of the ribosomal proteins, are coded by the chloroplast DNA. Although there have been numerous investigations into the genetics of algal chloroplasts, similar studies with higher plants have been hampered by the uniparental (maternal) pattern of transmission of chloroplasts observed in most species, and the shortage of suitable genetic markers (3,4).
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References
Dyer, T.A.(1985)The chloroplast genome and its products, in Oxford Surveys ofPlant Molecular and Ceil Biology,vol2 (Miflin, B. J., ed.), Oxford University Press, New York, pp. 147–177.
Borner, T.and Sears, B. B. (1986) Plastome Mutants. PIant Molecular Biology Reporfer 4, 69–92.
Maliga, P. (1986) Cell fusion to introduce genetic information coded by chloroplasts and mitochondria in flowering plants, in Molecular Development Biology Liss, New York, pp. 45–53.
Maliga, P. Breznovits, A.,and Márton, L. (1973) Streptomycin resistant plants from haploid callus culture of tobacco. Nature (New Biol.)244, 28–30.
Galun, E.and Aviv, D. (1983) Cytoplasmic hybridization: Genetic and breeding applications, in Handbook of Plant Cell Culture, vol 1 (Evans, D. A., Sharp, W. R., Ammirata, I’. V.,and Yamada, Y., eds.), Macmillan, New York, pp. 358–392.
Pelletier, G. (1986) Plant organelle genetics through somatic hybridization in Oxford Suroeys ofPlant MoZecularand Cell Biology, vol3 (Miflin, B. J., ed.), Oxford University Press, pp. 96–121.
Medgyesy, P., Fejes, E.,and Maliga, P. (1985) Interspecific chloroplast recombination in a Nicotiana somatic hybrid. PYOC. Natl. Acad. Sci. USA 82, 6960–6964.
Svab, Z.and Maliga, P. (1986) Nicotiana tabacum mutants with chloroplast encoded streptomycin resistance and pigment deficiency. Theor. AppZ. Gent. 72, 637–643.
Cs¬olÖ A.and Maliga, P. (1984) Large scale isolation of maternally inherited lincomycin resistance mutations in diploid Nicotiana plumbuginifolia protoplast cultures. Mol. Gen. Genet. 196, 407–412.
Cs¬olÖ, A., Medgyesy, P., Hideg, E., Demeter, S., Márton, L., and Maliga, P. (1985) Triazine resistant Nicotiana mutants from photomixotrophic cell cultures. Mol. Gen. Genet. 200, 508–510.
Fluhr, R., Aviv, D., Galun, E., and Edelman, M. (1985) Efficient induction and selection of chloroplast-encodedanitbiotic resitant mutantsin Nicotiana. Proc. Nutl. Acad. Sci. USA 82, 1485–1489.
Hagemann, R. (1982) Induction of plastome mutations by nitroso-urea-compounds, in Methods in Chloroplust Molecular Biology (Edelman, M., Hallick, R. B., and Chua, N. H., eds.), Elsevier Biomedical Press, Amsterdam, pp. 119–127.
Hosticka, L. P. and Hanson, M. R. (1984) Induction of plastid mutations in tomatoes by nitrosomethylurea. J. Hered. 75, 242–246.
Maliga, P. (1984) Cell culture procedures for mutant selection and characterization in Nicotiunu plumbaginifolia, in Cell Culture and Somatic Cell Genetics of Plants, vol1(Vasil, I. K., ed.), Academic, New York, pp. 552–562.
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McCabe, P.F., Cseplo, A., Timmons, A.M., Dix, P.J. (1990). Selection of Chloroplast Mutants. In: Pollard, J.W., Walker, J.M. (eds) Plant Cell and Tissue Culture. Methods in Molecular Biology™, vol 6. Humana Press. https://doi.org/10.1385/0-89603-161-6:467
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DOI: https://doi.org/10.1385/0-89603-161-6:467
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