Skip to main content

Selection of Chloroplast Mutants

  • Protocol
Plant Cell and Tissue Culture

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 6))

Abstract

The chloroplast genome encodes a number of proteins, including thylakoid proteins and the large subunit of ribulose biphosphate carboxylase, associated with the structure and function of the chloroplast (1-2). In addition, many components of the chloroplast translational machinery, such as all of the RNAs and some of the ribosomal proteins, are coded by the chloroplast DNA. Although there have been numerous investigations into the genetics of algal chloroplasts, similar studies with higher plants have been hampered by the uniparental (maternal) pattern of transmission of chloroplasts observed in most species, and the shortage of suitable genetic markers (3,4).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Dyer, T.A.(1985)The chloroplast genome and its products, in Oxford Surveys ofPlant Molecular and Ceil Biology,vol2 (Miflin, B. J., ed.), Oxford University Press, New York, pp. 147–177.

    Google Scholar 

  2. Borner, T.and Sears, B. B. (1986) Plastome Mutants. PIant Molecular Biology Reporfer 4, 69–92.

    Article  Google Scholar 

  3. Maliga, P. (1986) Cell fusion to introduce genetic information coded by chloroplasts and mitochondria in flowering plants, in Molecular Development Biology Liss, New York, pp. 45–53.

    Google Scholar 

  4. Maliga, P. Breznovits, A.,and Márton, L. (1973) Streptomycin resistant plants from haploid callus culture of tobacco. Nature (New Biol.)244, 28–30.

    Article  Google Scholar 

  5. Galun, E.and Aviv, D. (1983) Cytoplasmic hybridization: Genetic and breeding applications, in Handbook of Plant Cell Culture, vol 1 (Evans, D. A., Sharp, W. R., Ammirata, I’. V.,and Yamada, Y., eds.), Macmillan, New York, pp. 358–392.

    Google Scholar 

  6. Pelletier, G. (1986) Plant organelle genetics through somatic hybridization in Oxford Suroeys ofPlant MoZecularand Cell Biology, vol3 (Miflin, B. J., ed.), Oxford University Press, pp. 96–121.

    Google Scholar 

  7. Medgyesy, P., Fejes, E.,and Maliga, P. (1985) Interspecific chloroplast recombination in a Nicotiana somatic hybrid. PYOC. Natl. Acad. Sci. USA 82, 6960–6964.

    Article  CAS  Google Scholar 

  8. Svab, Z.and Maliga, P. (1986) Nicotiana tabacum mutants with chloroplast encoded streptomycin resistance and pigment deficiency. Theor. AppZ. Gent. 72, 637–643.

    Article  CAS  Google Scholar 

  9. Cs¬olÖ A.and Maliga, P. (1984) Large scale isolation of maternally inherited lincomycin resistance mutations in diploid Nicotiana plumbuginifolia protoplast cultures. Mol. Gen. Genet. 196, 407–412.

    Article  Google Scholar 

  10. Cs¬olÖ, A., Medgyesy, P., Hideg, E., Demeter, S., Márton, L., and Maliga, P. (1985) Triazine resistant Nicotiana mutants from photomixotrophic cell cultures. Mol. Gen. Genet. 200, 508–510.

    Article  Google Scholar 

  11. Fluhr, R., Aviv, D., Galun, E., and Edelman, M. (1985) Efficient induction and selection of chloroplast-encodedanitbiotic resitant mutantsin Nicotiana. Proc. Nutl. Acad. Sci. USA 82, 1485–1489.

    Article  CAS  Google Scholar 

  12. Hagemann, R. (1982) Induction of plastome mutations by nitroso-urea-compounds, in Methods in Chloroplust Molecular Biology (Edelman, M., Hallick, R. B., and Chua, N. H., eds.), Elsevier Biomedical Press, Amsterdam, pp. 119–127.

    Google Scholar 

  13. Hosticka, L. P. and Hanson, M. R. (1984) Induction of plastid mutations in tomatoes by nitrosomethylurea. J. Hered. 75, 242–246.

    CAS  Google Scholar 

  14. Maliga, P. (1984) Cell culture procedures for mutant selection and characterization in Nicotiunu plumbaginifolia, in Cell Culture and Somatic Cell Genetics of Plants, vol1(Vasil, I. K., ed.), Academic, New York, pp. 552–562.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1990 Humana Press Inc.

About this protocol

Cite this protocol

McCabe, P.F., Cseplo, A., Timmons, A.M., Dix, P.J. (1990). Selection of Chloroplast Mutants. In: Pollard, J.W., Walker, J.M. (eds) Plant Cell and Tissue Culture. Methods in Molecular Biology™, vol 6. Humana Press. https://doi.org/10.1385/0-89603-161-6:467

Download citation

  • DOI: https://doi.org/10.1385/0-89603-161-6:467

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-161-6

  • Online ISBN: 978-1-59259-493-1

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics