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Induction of Hairy Roots by Agrobacterium Rhizogenes and Growth of Hairy Roots In Vitro

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Plant Cell and Tissue Culture

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 6))

Abstract

The bacterial genus Agrobucterium includes two species of considerable interest to plant physiologists and pathologists alike. Infection by virulent strains of A. tumefaciens induces the formation of tumors and infection by A. rhizogenes the proliferation of roots in a wide range of dicotyledenous plants: crown-gall and hairy root diseases are not naturally recorded in monocotyledenous plants (1). Invasion of plant tissues by these free-living soil bacteria usually occurs at a wound site—possibly caused by insect or mechanical damage; nonvirulent strains of the bacteria may invade the plant, but only virulent strains give rise to symptoms of disease.

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References

  1. Bryant, J. A. (1988) Putting genes into plants. Plants Today 1,23–28.

    Google Scholar 

  2. Nester, E. W., Gordon, M. P., Amasino, R. M., and Yanofsky, M. F. (1984) Crowngall: a molecular and physiological analysis. Ann. Rev. Plant Physiol. 35,387–413.

    Article  CAS  Google Scholar 

  3. Weiler, E. W. and Schroder, J. (1987) Hormone genes and crown gall disease. Trends in Biochemical Sciences 12,271–275.

    Article  CAS  Google Scholar 

  4. Birot, A. M., Bouchez, D., Casse-Delbart, F., Durand-Tardif, M., Jouanin, L., Pautot, V., Robaglia, C., Tepfer, D., Tepfer, M., Tourneur, J., and Vilaine, F. (1987) Studies and uses of the Ri plasmids of Agrobacterium rhizogenes. Plant Physiol. Biochem. 25 (3), 323–335.

    CAS  Google Scholar 

  5. Tepfer, M. and Casse-Delbart, F. (1987) Agrobucterium rhizogenes as a vector for transforming higher plants. Microbiological Sciences 4 1, 24–28.

    PubMed  CAS  Google Scholar 

  6. Huffman, G. A., White, F. F., Gordon, M. P., and Nester, E. W. (1984) Hairy root inducing plasmid: Physical map and homology to tumor-inducing plasmids. J. Bacteriol. 157,269–276.

    PubMed  CAS  Google Scholar 

  7. Offringa, I. A., Melchers, L. S., Regensburg-Tuink, A. J. G., Costantino, P., Schilperoot, R. A., and Hooykaas, P. J. J. (1986) Complementation of Agrobacterium tumefaciens tumor-inducing aux mutants by genes from the TR-region of the Ri plasmid of Agrobacterium rhizogenes. Proc. Natl. Ad. Sci. USA 83,6935–6939.

    Article  CAS  Google Scholar 

  8. Hamill, J. D., Parr, A. J., Rhodes, M. J. C., Robins, R. J., and Walton, N. J. (1987) New routes to plant secondary products. Biotechnology 5,800–804.

    Article  CAS  Google Scholar 

  9. Perani, L., Radke, S., Wilke-Douglas, M., and Bossert, M. (1986) Gene transfer methods for crop improvement: introduction of foreign DNA into plants. Physiol. Plantarum 68,566–570.

    Article  CAS  Google Scholar 

  10. Comai, L., Facciotti, D., Hiatt, W. R., Thompson, G., Rose, R. E., and Stalker, D. M.(1985) Expression in plants of a mutant are gene from Salmonella typhimurium confers tolerance to glyphosate. Nature 317,741–744.

    Article  CAS  Google Scholar 

  11. Jensen, J. S., Marcker, K. A., Otten, L., and Schell, J. (1986) Nodule-specific expression of a chimaeric soybean leghaemoglobin gene in transgenic Lotus corniculutus. Nature 322699–674.

    Google Scholar 

  12. Sukhapinda, K., Trulson, A. J., Shahin, E. A., and Simpson, R. B. (1987) Phenotype of plants derived from hairy roots transformed with Agrobucterium rhizogenes, in Molecular Biology of Plant Growth Control (Fox, J. E. and Jacobs, M., eds.), Liss, New York, pp. 381–389.

    Google Scholar 

  13. Mugnier, J. (1988) Establishment of new axenic hairy root lines by inoculation with Agrobucterium rhizogenes. Plant Cell Reports 7,9–12.

    Article  Google Scholar 

  14. Flores, H. E., Hoy, M. W., and Pickard, J. J. (1987) Secondary metabolites from root cultures. Trends in Biotechnology 5,64–69.

    Article  CAS  Google Scholar 

  15. Payne, J., Hamill, J. D., Robins, R. J., and Rhodes, M. J. C. (1987) Production of hyoscyamineby “hairy-root” culturesof Duturustramonium. Planta medica 53 (5),474–478.

    Article  PubMed  CAS  Google Scholar 

  16. Fujita, Y. and Tabata, M. (1987) Secondary metabolites from plant cells—pharmaceutical applications and progress in commercial production, in Plant Tissueand Cell Culture (Green, C. E., Somers, D. A., Hackett, W. P., and Biesboer, D. D., eds.), Liss, New York, pp. 169–185.

    Google Scholar 

  17. Charlwood, B., Brown, J. T., Moustou, C., and Charlwood, K. A. (1988) Pelargoniurns: flavours, fragrances and the new technology. Plants Today 2,42–46.

    Google Scholar 

  18. Chandler, S. and Dodds, J. (1983) Solasodine production in rapidly proliferating tissue cultures of Solanum laciniutum Ait. Plant Cell Reports 2,69–72.

    Article  CAS  Google Scholar 

  19. Anderson, L. A., Keene, A. T., and Phillipson, J. D. (1982) Alkaloid production by leaf organ, root organ and cell suspension cultures of Cinchona ledgeriana. Planta medica 46,25–27.

    Article  PubMed  CAS  Google Scholar 

  20. Jung, G. and Tepfer, D. (1987) Use of genetic transformation by the Rt T-DNA of Agrobucterium rhizogenes to stimulate biomass and tropane alkaloid production in Atropa belladonna and Calystegia sepium roots grown in vitro. Plant Science 50 145–151.

    Article  CAS  Google Scholar 

  21. Parr, A. J. and Hamill, J. D. (1987) Relationship between Agrobucterium rhizogenes transformed hairy roots and intact uninfected Nicotiana plants. Phytochemistry 26, 3241–3245.

    Article  CAS  Google Scholar 

  22. Robins, R. J., Hamill, J. D., Parr, A. J., Smith, K., Walton, N. J., and Rhodes, M. J. C. (1987) Potential for use of nicotinic acid as a selective agent for isolation of high nicotine-producing lines of Nicotiana rustica hairy root culture. Plant Cell Reports 6,122–126.

    CAS  Google Scholar 

  23. Tepfer, D. (1984) Transformation of several species of higher plants by Agrobucterium rhizogenes: sexual transmission of the transformed genotype and phenotype. Cell 37,959–967.

    Article  PubMed  CAS  Google Scholar 

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© 1990 Humana Press Inc.

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Hunter, C.S., Neill, S.J. (1990). Induction of Hairy Roots by Agrobacterium Rhizogenes and Growth of Hairy Roots In Vitro. In: Pollard, J.W., Walker, J.M. (eds) Plant Cell and Tissue Culture. Methods in Molecular Biology™, vol 6. Humana Press. https://doi.org/10.1385/0-89603-161-6:279

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  • DOI: https://doi.org/10.1385/0-89603-161-6:279

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-161-6

  • Online ISBN: 978-1-59259-493-1

  • eBook Packages: Springer Protocols

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