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Plant Cell and Tissue Culture pp 227–236Cite as

The Preparation of Micropropagated Plantlets for Transfer to Soil Without Acclimatization

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 6))

Abstract

Plantlets cultured in vitro on agar-based media in a water-saturated atmosphere wilt rapidly when transferred to normal greenhouse or field conditions. Water is rapidly lost from the leaves because stomata fail to respond to those stimuli that normally induce closure (1-4), and poor development of epicuticular wax results in loss of water through the cuticle (5-7). Uptake of water by the roots is limited by damage incurred during transplantation and by poor contact with the substrate. Problems of transplantation are accentuated in vitrified plantlets, which grow slowly and wilt rapidly. Reduced deposition of cellulose and lignin in these plantlets causes reduced cell wall pressure, leading to increased water uptake by the cells and a glassy turgescence of leaves and stems (8,9).

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Author information

Authors and Affiliations

  1. North East London Polytechnic, London, UK

    Andrew V. Roberts, Elaine F. Smith & John Mottley

Authors
  1. Andrew V. Roberts
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  2. Elaine F. Smith
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  3. John Mottley
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Editor information

Editors and Affiliations

  1. Albert Einstein College of Medicine, Bronx, New York

    Jerey W. Pollard

  2. The Hatfield Polytechnic, Hatfield, Hertfordshire, UK

    John M. Walker

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© 1990 Humana Press Inc.

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Roberts, A.V., Smith, E.F., Mottley, J. (1990). The Preparation of Micropropagated Plantlets for Transfer to Soil Without Acclimatization. In: Pollard, J.W., Walker, J.M. (eds) Plant Cell and Tissue Culture. Methods in Molecular Biology™, vol 6. Humana Press. https://doi.org/10.1385/0-89603-161-6:227

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  • DOI: https://doi.org/10.1385/0-89603-161-6:227

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-161-6

  • Online ISBN: 978-1-59259-493-1

  • eBook Packages: Springer Protocols

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