Abstract
This chapter will deal with methods of clonal propagation for members of the two major morphological groups of orchids. The first group, sympodials, includes such genera as Cymbidium, Cattleya, Dendrobium, and Oncidium. They are characterized by a multi-branchingrhizome that can supply an abundance of axillary shoots for use as explants. They were among the first orchids to be successfully propagated, and techniques for their in vitro initiation (i.e., establishment) and subsequent proliferation are well established (1-8). The second group, monopodials, include Phaluenopsis and Vanda, and are characterized by a single, unbranched axis of growth that possesses few readily available axillary shoots for use as explants. Significantly different in their morphologies, the two groups require different approaches to explant selection and subsequent culturing. The successful large scale micropropagation of monopodials is, in fact, a relatively recent achievement (9): the culmination of a wide variety of studies using different media compositions and supplements (10-18).
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References
Churchill, M. E. (1973) Tissue culture of orchids. New Phyfol. 72, 161–166.
Lindemann, E. G. P. (1970) Meristemculture of Cuttleya. Amer. Orchid Soc. Bull. 39, 1002–1004.
Morel, G. (1960) Producing virus free Cymbidiums. Amer. Orchid Soc. Bull. 29, 495–497.
Morel, G. (1964) A new means of clonal propagation of orchids. Amer. Orchid Soc.Bull. 33, 473–478.
Sagawa, Y. (1966) Clonal propagation of Cymbidiums through shoot meristem culture. Amer. Orchid Soc. Bull. 35, 188–192.
Sagawa, Y. (1967) Clonal propagation of Dendrobiums through shoot meristern culture. Amer. Orchid Soc. Bull. 36, 856–859.
Scully, R. (1967) Aspects of meristem culture in the Cattleya alliance. Amer. Orchid Soc. Bull. 36, 103–108.
Wimber, D. (1963) Clonal multiplication of Cymbidiums through tissue culture of the shoot meristem. Amer. Orchid Soc. Bull. 32, 105–107.
Kuhn, L. (1982) Orchid propagation in the EYMC. Marie Selby Bot. Gard. Bull. 9, 32–34.
Griesbach, R. J. (1983) The use of indoleacetylamino acids in the in vitro propagation of Phalaenopsis orchids. Sci. Hort. 19, 363–366.
Intuwong, O. (1974) Clonal propagation of Phalaenopsis by shoot tip culture. Amer. Orchid Soc. Bull. 43, 893–895.
Huang, L. C. (1984) Alternative media and method for Cuttleya propagation by tissue culture. Amer. Orchid Soc. Bull. 53, 167–170.
Rotor, G. (1949) A method of vegetative propagation of Phalaenopsis species and hybrids. Amer. Orchid Soc. Bull. l8, 738,739.
Sagawa, Y. (1961) Vegetative propagation of Phalaenopsis stem cuttings. Amer. Orhid Soc. Bull. 30, 803–809.
Scully, R. (1966) Stem propagation of Phalaenopsis. Amer. Orchid Soc. Bull. 35, 40–42.
Lay, M. and Fan, F. (1978) Studies on the tissue culture of orchids. Orchid Rev. Oct, 308–310.
Tanaka, M. (1977) Clonal propagation of Phalaenopsis by leaf tissue culture. Amer. Orchid Soc. Bull. 46, 733–737.
Zimmer, K. and Pieper, W. (1978) Clonal propagation of Phalaenopsis by excised buds. Orchid Rev. July, 223–227.
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© 1990 Humana Press Inc.
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Jones, D., Tisserat, B. (1990). Clonal Propagation of Orchids. In: Pollard, J.W., Walker, J.M. (eds) Plant Cell and Tissue Culture. Methods in Molecular Biology™, vol 6. Humana Press. https://doi.org/10.1385/0-89603-161-6:181
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DOI: https://doi.org/10.1385/0-89603-161-6:181
Publisher Name: Humana Press
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