Enzyme Extraction and Assessment of Enzyme Production

  • Debbie Grey
Part of the Methods in Molecular Biology™ book series (MIMB, volume 6)


There have been numerous reports of enzyme synthesis in cultured plant cells and the presence of certain enzymes in the culture medium. Most of these studies have been performed in the context of the characterization of these enzymes from cell cultures. However, cell cultures-in particular those secreting enzymes into the medium-can be used as a process system for enzyme production. Surprisingly, there is very little information regarding the potential of plant cell cultures as a tool for enzyme productivity.


Extraction Buffer Plant Cell Culture Extracellular Enzyme Activity Enzyme Activity Unit Graduated Test Tube 
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  1. 1.
    Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of micro-gram quantities of protein utilizing the principle of protein-dye binding. Ad. Biochem. 72, 24-254.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 1990

Authors and Affiliations

  • Debbie Grey
    • 1
  1. 1.Wolson Institute of BiotechnologySheieldUK

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