Selection of Chloroplast Mutants

  • Paul F. McCabe
  • Agnes Cseplo
  • Aileen M. Timmons
  • Philip J. Dix
Part of the Methods in Molecular Biology™ book series (MIMB, volume 6)


The chloroplast genome encodes a number of proteins, including thylakoid proteins and the large subunit of ribulose biphosphate carboxylase, associated with the structure and function of the chloroplast (1-2). In addition, many components of the chloroplast translational machinery, such as all of the RNAs and some of the ribosomal proteins, are coded by the chloroplast DNA. Although there have been numerous investigations into the genetics of algal chloroplasts, similar studies with higher plants have been hampered by the uniparental (maternal) pattern of transmission of chloroplasts observed in most species, and the shortage of suitable genetic markers (3,4).


Shoot Culture Streptomycin Sulfate Protoplast Culture Leaf Strip Albino Mutant 
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Copyright information

© Humana Press Inc. 1990

Authors and Affiliations

  • Paul F. McCabe
    • 1
  • Agnes Cseplo
    • 2
  • Aileen M. Timmons
    • 1
  • Philip J. Dix
    • 1
  1. 1.St. Patrick’s CollegeKildareRepublic of Ireland
  2. 2.Hungarian Academy of SciencesSzegedHungary

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