Abstract
As other chapters in this book demonstrate, intra- and extracellular recording provide considerable information about the electrical behavior of cells. However, as Hodgkin and Huxley (1952) taught us long ago, analysis of the mechanisms that generate electrical activity is far easier with voltage clamp. If the behavior of ion channels depends on voltage, the first step in studying such channels is to control the voltage. Under voltage clamp, the kinetic behavior of a channel (or of a macroscopic assemblage of channels) is at its simplest. Without voltage clamp, there is a complex interaction between channel gating and membrane potential. When channels open, current through them affects the membrane potential, which in turn makes channels open (or close), affecting the membrane potential....
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References
Adams P. R., Brown D. A., and Constanti A. (1982a) M-currents and other potassium currents in bullfrog sympathetic neurones. J. Phystol. 330, 537–572.
Adams P. R., Constanti A., Brown D. A., and Clark R. B. (1982b) Intracellular Ca2+ activates a fast voltage-sensitive K+ current in vertebrate sympathetic neurones. Nature (Lond). 296, 746–749.
Adams P. R., Jones S. W., Pennefather P., Brown D. A., Koch C., and Lancaster B. (1986) Slow synaptic transmission in frog sympathetic ganglia. J. Exp. Blol. 124, 259–285.
Armstrong C. M. and Chow R. H. (1987) Supercharging: A method for improving patch-clamp performance. Biophys. J. 52, 133–136.
Cachelin A. B. and Rice P. D. (1987) Microcomputers in the laboratory, in Microelectrode Techniques. The Plymouth Workshop Handbook (Standen N. B., Gray P. T. A., and Whitaker M. J., eds.), pp. 229–248, The Company of Biologists, Cambridge, England.
DiFrancesco D., Ferrom A., Visentin S., and Zaza A. (1985) Cadmium-induced blockade of the cardiac fast Na channels in calf Purkinle fibres. Proc. Roy. Sot. B 223, 475–484.
Finkel A. S. and Gage P. W. (1985) Conventional voltage clamping with two intracellular microelectrodes, in Voltage and Patch Clumping with Microelectrodes (Smith T. G. Jr., Lecar H., Redman S. J., and Gage P. W., eds.), American Physiological Society, Bethesda, Maryland, pp. 47–94.
Finkel A. S. and Redman S. (1984) Theory and operation of a single microelectrode voltage clamp. J. Neurosci. Meth. 11, 101–127.
Finkel A. S. and Redman S. J. (1985) Optimal voltage clamping with single microelectrode, in Voltage and Patch Clamping with Microelectrodes (Smith T. G., Jr., Lecar H., Redman S. J., and Gage P. W., eds.), American Physiological Society, Bethesda, Maryland, pp. 95–120.
Halliwell J. V. and Adams P. R. (1982) Voltage-clamp analysis of muscarmic excitation in hippocampal neurons. Bruin Res. 250, 71–92.
Hamill O. P. (1983) Potassium and chloride channels in red blood cells, in Single Channel Recording (Sakmann B. and Neher E., eds.), Plenum New York, pp. 451–471.
Hamill O. P., Marty A., Neher E., Sakmann B., and Sigworth F. J, (1981) Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch. 391, 85–100.
Hille B. (1984) Ionrc Channels of Excitable Membranes (Sinauer Associates, Sunderland, Massachusetts
Hodgkin A. L. and Huxley A. F. (1952) A quantitative description of membrane current and its application to conduction and excitation in nerve. J. Physiol. 117, 500–544.
Horn R. and Marty A (1988) Muscarinic activation of ionic currents measured by a new whole-ceil recording method. J. Gen. Physiol. 92, 145–159.
Ikeda S. R., Schofield G. G, and Weight F. F. (1986) Na+ and Ca2+ currents of acutely isolated adult rat nodose ganglion cells. J. Neurophysiol. 55, 527–539.
Jack J. J. B., Noble D., and Tsien R. W. (1975) Elecfrtc Current Flow in Excitable Cells (Oxford University Press, Oxford, England).
Johnston D. and Brown T. H. (1983) Interpretation of voltage-clamp measurements in hippocampal neurons. J. Neurophysiol. 50, 464–486.
Jones S. W. (1987) Sodium currents in dissociated bull-frog sympathetic neurones. J. Physlol. 389, 605–627.
Jones S. W (1989) On the resting potential of isolated frog sympathetic neurons. Neuron 3, 153–161.
Jones S. W. and Marks T. N. (1989) Calcium currents in bullfrog sympathetic neurons. I. Activation kinetics and pharmacology. J, Gen. Physiol. 94, 151–167.
Kostyuk P. G., Veselovsky N. S., and Tsyndrenko A. Y. (1981) Ionic currents in the somatic membrane of rat dorsal root ganglion neurons—I. Sodium currents. Neuroscience 6, 2423–2430.
Kramer R. H. (1986) Axonal contribution to subthreshold currents in Aplysia busting pacemaker neurons. Cell. Molec. Neurobiol. 6, 239–253.
Lancaster B. and Pennefather P. (1987) Potassium currents evoked by brief depolarizations in bull-frog sympathetic ganglion cells. J. Physiol. 387, 519–548.
Lipscombe D., Madison D. V., Poenie M., Reuter H., Tsien R. Y., and Tsien R. W. (1988) Spatial distribution of calcium channels and cytosolic calcium transients in growth cones and cell bodies of sympathetic neurons. Proc. Natl. Acad Sci. USA 85, 2398–2402.
Madison D. V., Malenka R. C., and Nicoll R. A. (1986) Phorbol esters block a voltage-sensitive chloride current in hippocampal pyramidal cells. Nature 321, 695–697.
Marty A. and Neher E. (1985) Potassium channels in cultured bovine adrenal chromaffin cells. J. Phystol. 367, 117–141.
Moore J. W. (1971) Voltage clamp methods, in Biophysics and Physiology of Excifuble Membranes (Adelman W. J., ed.), Van Nostrand, New York, pp. 143–167.
Moore J. W. (1985) Comparison of voltage clamps with microelectrode and sucrose-gap techniques, in Voltage and Patch Clamping with Mic-roelectrodes (Smith T. G., Jr., Lecar H., Redman S. J., and Gage P. W., eds.), American Physiological Society, Bethesda, Maryland, pp. 217–230.
Pennefather P., Lancaster B., Adams I. R., and Nicoll R. A. (1985) Two distinct Ca-dependent K currents in bullfrog sympathetic ganglion cells. Proc. Natl. Acad. Sci. USA 82, 3040–3044.
Rall W. and Segev I. (1985) Space-clamp problems when voltage clamping branched neurons with intracellular microelectrodes, in Voltage and Patch Clumping with Microelectrodes (Smith T. G., Jr., Lecar H., Redman S. J., and Gage P. W., eds.), American Physiological Society, Bethesda, Maryland, pp. 191–215.
Sakmann B. and Neher E. (eds.) (1983) Single-Channel Recording. (Plenum, New York).
Sigworth F. J. (1983) Electronic design of the patch clamp, in Single-Channel Recording (Sakmann B. and Neher E., eds.), Plenum, New York, pp. 3–35.
Smith T. G., Jr., Lecar H., Redman S. J., and Gage P. W. (eds.) (1985) Voltage and Patch Clumping with Microelectrodes (American Physiological Society, Bethesda, Maryland).
Standen N. B., Gray I. T. A., and Whitaker M. J. (eds.) (1987) Microelectrade Techniques. The Plymouth Workshop Handbook (The Company of Biologists, Cambridge, England).
Wilson W. A., and Goldner M. M. (1975) Voltage clamping with a single microelectrode, J. Neurobiol. 6, 411–422.
Wonderlin W. F., French R. J., and Arispe N. J. (1989) Recording and analysis of currents from single ion channels, in Neuromethods, Vol. 14 (Boulton A. A., Baker G. B., and Vanderwolf C. H., eds.), Humana Press, Clifton, New Jersey, in press.
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Jones, S.W. (1990). Whole-Cell and Microelectrode Voltage Clamp. In: Boulton, A.A., Baker, G.B., Vanderwolf, C.H. (eds) Neurophysiological Techniques. Neuromethods, vol 14. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-160-8:143
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