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Animal Cell Culture pp 43–47Cite as

Establishment of Lymphoblastoid Cell Lines

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 5))

Abstract

The ability to establish long-term B lymphocyte cultures from patients carrying particular genetic characteristics or with the ability to secrete specific antibodies (1) is an extremely valuable technique. However, there are several basic principles to follow in the approach to this technology. It is the purpose of this chapter to provide all the information necessary to run an efficient and safe Epstein-Barr Virus (EBV) transformation system. More detailed information regarding the use of this technique for the preparation of human monoclonal antibodies is given elsewhere (2).

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References

  1. Steinitz, M., Klein, G., Koskimies, S., and Makel, O. (1977) EB virus-induced B lymphocyte cell lines producing specific antibody. Nature 269, 420.

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  2. Crawford, D. H. (1986) Use of the virus to prepare human-derived monoclonal antibodies, in The Epstein-Barr Virus: Recent Advances (Epstein, M. A. and Achong, B. G., eds.), William Heinemann Medical Books, London, p. 249.

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  3. Adams, A. (1975) EBV Production, Concentration and Purification (Ablushi, D. V., Aalesed, H. G., and De The, G., eds.), IARC, Lyon, France, p. 129.

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  4. Miller, G. and Lipman, M. (1973) Release of infectious Epstein-Barr virus by transformed marmoset leucocytes. Proc. Natl. Acad. Sci.USA 70, 190.

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Author information

Authors and Affiliations

  1. European Collection of Animal Cell Cultures, PHLS CAMR, Salisbury, UK

    A. Doyle

Authors
  1. A. Doyle
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Editor information

Editors and Affiliations

  1. Albert Einstein College of Medicine, Bronx, New York

    Jeffrey W. Pollard

  2. The Hatfield Polytechnic, Hatfield, Hertsfordshire, UK

    John M. Walker

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© 1990 The Humana Press Inc.

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Doyle, A. (1990). Establishment of Lymphoblastoid Cell Lines. In: Walker, J.M., Pollard, J.W., Walker, J.M. (eds) Animal Cell Culture. Methods in Molecular Biology, vol 5. Humana Press. https://doi.org/10.1385/0-89603-150-0:43

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  • DOI: https://doi.org/10.1385/0-89603-150-0:43

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-150-0

  • Online ISBN: 978-1-59259-492-4

  • eBook Packages: Springer Protocols

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