Abstract
The so-called hematopoietic stem cells from mouse and human bone marrow can be induced in vitro to form colonies, provided that the appropriate hormones (colony stimulating factors, CSFs) and culture conditions have been selected. Such hematopoietic colony cultures are of interest for in vitro cytotoxicity testing of a great number of drugs. In fact, it has been increasingly recognized that serious side effects of many drugs involve injury to hematopoietic organs. Moreover, several hormones and inhibitors (including chalones) that regulate hematopoiesis have attracted clinical interest and their identity can be determined by such in vitro cultures. Therefore hematopoietic stem cells seem to provide identified targets to assay for either stimulatory, inhibitory, or cytotoxic drug effects.
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References
Metcalf, D. (1984) The Hemopoietic Colony Stimulating Factors (Elsevier, Amsterdam).
Salmon, S. E. and Trent, J. M. (eds.) (1984) Human Tumor Cloning (Grune & Stratton, Orlando, Florida).
Courtenay, V. D. and Mills, J. (1978) An in vitro colony assay for human tumors grown in immune-suppressed mice and treated in vivo with cytotoxic agents. Br. J. Cancer 37, 261–268.
Maurer, H. R. (1979) In vitro colony growth of granulocytes, macrophages, T-and B-lymphocytes in agar capillaries. Acta Haematol. 62, 322–325.
Maurer, H. R. and Henry, R. (1976) Automated scanning of bone marrow cell colonies growing in agar-containing glass capillaries. Exp. Cell Res. 103, 271–277.
Echarti, C., and Maurer, H. R. (1989) Manuscript in preparation.
Maurer, H. R. and Henry, R. (1976) Colony growth of mouse bone marrow cells in agar contained in glass capillaries. Blut 33, 11–22.
Maurer, H. R., Maschler, R., Dietrich, R., and Goebel, B. (1977) In vitro culture of lymphocyte colonies in agar capillary tubes after PHA-stimulation. J. Immunol. 18, 353–364.
Maurer, H. R. and Ali-Osman, F. (1981) Tumor stem cell cloning in agar-containing capillaries. Naturwissenschaften 68, 381–383.
Maurer, H. R. and Henry, R. (1978) Drug evaluation on haemopoietic cells in vitro. Arzneimittel-Forsch. (Drug Res.) 28, 601–605.
Maurer H. R., Henry, R., and Maschler, R. (1978) Chalone inhibition of granulocyte colony growth in agar: Kinetic quantitation by capillary tube scanning. Cell Tiss. Kinet. 11, 129–138.
Von Hoff, D. D., Forseth, B. J., Huong, M., Buchok, J. B., and Lathan, B. (1986) Improved plating efficiencies for human tumors cloned in capillary tubes versus Petri dishes. Cancer Res. 46, 4012–4017.
Neumeier, R., Maurer, H. R., Maschler, R., and Bombik, B. M. (1981) A micromethod to culture granulocyte colonies from human bone marrow in agar-containing glass capillaries. Exp. Hematol. 9, 44–51.
Maschler, R. and Maurer, H. R. (1978) Screening for specific calf thymus inhibitors (chalones) by different assays. Hoppe-Seyler’s Z. Physiol. Chem. 359, 825–834.
Tortarolo, M., Braun, R., Hübner, G. E., and Maurer, H. R. (1982) In vitro effects of epoxide bearing valepotriates on mouse early hematopoietic progenitor cells and human T-lymphocytes. Arch. Toxicol. 51, 37–42.
Matthiessen, P., Echarti, C., Gerber, J., and Maurer, H. R. (1978) T-lymphocyte colony formation of murine thymocytes in agar contained in glass capillaries. Blut 55, 517–522.
Ulmer, A. and Maurer, H. R. (1978) The formation of B-lymphocyte colonies in agar-containing glass capillaries. Immunology 34, 919–925.
Ali-Osman, F., Bier, J., and Maurer, H. R. (1983) In vitro cytostatic drug sensitivity testing in the human tumor stem cell assay: a modified method for the determination of the sensitivity index. Tumor Diagn. Ther. 4, 1–6.
Ali-Osman, F., Giblin, J., Dougherty, D., and Rosenblum, M. L. (1987) Application of in vivo and in vitro pharmacokinetics for physiologically relevant drug exposure in a human tumor clonogenic cell assay. Cancer Res. 47, 3718–3724.
Sheridan, J. W. and Metcalf, D. (1973) A low molecular weight factor in lung-conditioned medium stimulating granulocyte and monocyte formation in vitro. ]. Cell. Physiol. 81, 11–24.
Leibovits, A., Liu, R., Hayes, C., and Salmon, S. E. (1983) A hypo-osmotic medium to disaggregate tumor cell clumps into viable and clonogenic single cells for the human tumor stem cell clonogenic assay. Internat. J. Cell Cloning 1, 478–485.
Maschler, R. and Maurer, H. R. (1981) Lymphocyte chalone from calf thymus: problems of large scale extraction and assay. Z. Naturforsch. 36 c, 568–571.
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Maurer, H.R., Echarti, C. (1990). Clonogenic Assays for Hematopoietic and Tumor Cells Using Agar-Containing Capillaries. In: Walker, J.M., Pollard, J.W., Walker, J.M. (eds) Animal Cell Culture. Methods in Molecular Biology, vol 5. Humana Press. https://doi.org/10.1385/0-89603-150-0:379
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DOI: https://doi.org/10.1385/0-89603-150-0:379
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