Abstract
Erythroid progenitor cells have been classified into three groups of increasing maturity: the primitive burst forming unit (p-BFU-E), the mature burst forming unit (m-BFU-E), and the erythropoietin responsive colony forming unit (CFU-E). This classification is based on their time of maturation in vitro, their proliferative capacity, and their responsiveness to growth factors (1). The CFU-E can be distinguished from the more primitive erythroid progenitors by their ability to proliferate and mature in response to a single growth factor, erythropoietin. In clonal assays in vitro, the CFU-E form single or double clusters characteristically containing 8–64 mature or maturing erythroid cells 2 d after cultures have been initiated with mouse bone marrow or fetal liver (2,3).
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© 1990 The Humana Press Inc.
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Watt, S.M., Davis, J.M. (1990). Flow Sorting for Isolating CFU-E. In: Walker, J.M., Pollard, J.W., Walker, J.M. (eds) Animal Cell Culture. Methods in Molecular Biology, vol 5. Humana Press. https://doi.org/10.1385/0-89603-150-0:347
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DOI: https://doi.org/10.1385/0-89603-150-0:347
Publisher Name: Humana Press
Print ISBN: 978-0-89603-150-0
Online ISBN: 978-1-59259-492-4
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