Abstract
Agar culture systems for the clonal growth and differentiation of hemopoietic cells were first described 20 yr ago (1). The progenitor cells that developed into colonies in agar after several days of culture in the presence of a source of hemopoietic growth factor (2,3) were initially called “Colony Forming Units in Culture” (CFU-C). They are found in bone marrow, spleen, blood, fetal liver, and yolk sac. It was subsequently demonstrated that the CFU-C population was heterogeneous and contained progenitors giving rise to granulocyte/macrophage (CFU-GM), granulocyte (CFU-G), and macrophage (CFU-M) colonies. Progenitor cells of other lineages (erythroid and megakaryocytic) have also been similarly demonstrated in hemopoietic organs.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Bradley, T. R. and Metcalf, D. (1966) The growth of mouse bone marrow cells in vitro. Austalian Journal of Experimental Biology and Medical Science 44, 287–299.
Metcalf, D. (1986) The molecular biology and functions of the granulocyte macro-phage-stimulating factors. Blood 67, 257–264.
Clark, S. C. and Kamen, R. (1987) The human hematopoietic colony stimulating factors. Science 236, 1229–1237.
Boyum, A. (1968) Isolation of leukocytes from human blood. Scandinavian Journal of Clinical and Laboratory Investigation 21:Supp. 97, 31–50.
Bradley, R. R., Hodgson, G. S., and Rosendaal, M. (1987) The effects of oxygen tension on haemopoietic and fibroblast cell proliferation in vitro. J. Cell Physiol. 94, 517–522.
Lanotte, M., Schor, S., and Dexter, T. M. (1981) Collagen gels as a matrix for haemopoiesis. Journal of Cellular Physiology 106,269–277.
Schor, S. L. and Court, J. (1979) Different mechanisms in the attachment of cells to native and denatured collagen. Journal of Cell Science 38, 267–281.
Lanotte, M. (1984) Terminal differentiation of hemopoietic cell clones cultured in tri-dimensional collagen matrix in situ cell morphology and enzyme histochemistry analysis. Biology of the Cell 50, 107–120.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 The Humana Press Inc.
About this protocol
Cite this protocol
Praloran, V., Bartocci, A. (1990). CFU-C in Agar. In: Walker, J.M., Pollard, J.W., Walker, J.M. (eds) Animal Cell Culture. Methods in Molecular Biology, vol 5. Humana Press. https://doi.org/10.1385/0-89603-150-0:323
Download citation
DOI: https://doi.org/10.1385/0-89603-150-0:323
Publisher Name: Humana Press
Print ISBN: 978-0-89603-150-0
Online ISBN: 978-1-59259-492-4
eBook Packages: Springer Protocols