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Animal Cell Culture pp 237–276Cite as

Defining Hormone and Matrix Requirements for Differentiated Epithelia

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 5))

Abstract

The culture of differentiated cells requires conditions that acknowledge the complicated cell-cell interactions that both occur in vivo and are responsible for affecting and maintaining the differentiated states of cells. In brief, one must use conditions that mimic the epithelial-mesenchymal relationship that is universal and constitutes the organizational basis for all metazoan tissues. This relationship is sustained by a set of soluble signals (autocrine, paracrine, and endocrine) and by a set of insoluble signals (the extracellular matrix). Since this is a technical and methodological article, neither the scientific evidence for the importance of the epithelial-mesenchymal relationship nor the evidence forming the basis for the culture conditions will be described. Recent reviews have discussed this background in considerable detail (1–12

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Authors and Affiliations

  1. Albert Einstein College of Medicine, Bronx, New York

    Lola M. Reid

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  1. Lola M. Reid
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  1. Albert Einstein College of Medicine, Bronx, New York

    Jeffrey W. Pollard

  2. The Hatfield Polytechnic, Hatfield, Hertsfordshire, UK

    John M. Walker

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Reid, L.M. (1990). Defining Hormone and Matrix Requirements for Differentiated Epithelia. In: Walker, J.M., Pollard, J.W., Walker, J.M. (eds) Animal Cell Culture. Methods in Molecular Biology, vol 5. Humana Press. https://doi.org/10.1385/0-89603-150-0:237

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  • DOI: https://doi.org/10.1385/0-89603-150-0:237

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-150-0

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