Abstract
Hormonally defined serum-free media have been developed for growth and functional studies with kidney epithelial cell cultures. Not only can several established kidney epithelial cell lines (MDCK and LLC-PK1) be grown in a serum-free environment (1,2), but in addition, primary cultures of kidney epithelial cells can also be grown serum free (1–4). Investigations with primary kidney cell cultures are advantageous for several reasons. First of all, kidney cells can be grown in vitro from the animal of choice. Thus, the results of tissue culture studies can be more closely correlated with animal studies. Secondly, new tissue culture systems can be developed that more closely resemble kidney cells in vivo than those obtained with established kidney cell lines.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Taub, M., Chuman, L., Saier, M. H., and Sato, G. (1979) Growth of Madin Darby canine kidney epithelial cell (MDCK) line in hormone-supplemented serum-free medium. Proc. Natl. Acad. Sci. USA, 76, 3338–3342.
Chuman, L., Fine, L. G., Cohen, A. I., and Saier, M. H. (1982) Continuous growth of proximal tubular epithelial cells in hormone-supplemented serum-free medium. J. Cell Biol. 94, 506–510.
Taub, M. and Sato, G. (1979) Growth of functional primary cultures of kidney epithelial cells in defined medium. J. Cell. Physiol. 105, 369–378.
Chung, S. D., Alavi, N., Livingston, D., Hiller, S., and Taub, M. (1982) Characterization of primary rabbit kidney cultures that express proximal tubule functions in a hormonally defined medium. J. Cell. Biol. 95, 118–126.
Taub, M. (1985) Primary culture of proximal tubule cells in defined medium. J. Tissue Cult. Methods 9, 67–72.
Waqar, M. A., Seto, J., Chung, S. D., Hiller-Grohol, S., and Taub, M. (1985) Phosphate uptake by primary renal proximal tubule cells grown in hormonally defined medium. J. Cell. Physiol. 124, 411–423.
Brendel, K. and Meezen, E. (1975) Isolation and properties of a pure preparation of proximal kidney tubules obtained without collagenase treatment. Fed. Proc. 34, 803.
Meezan, E. K., Brendel, J., Ulreich, J., and Carlson, E. C. (1973) Properties of a pure metabolically active glomerular preparation from rat kidneys. I. Isolation. J. Pharmacol. Exp. Ther. 187, 332–341.
Sakhrani, L. M., Badie-Dezfooly, B., Trizna, W., Mikhail, K, Lowe, A.C., Taub, M., and Fine, L. G. (1987) Transport and metabolism of glucose by renal proximal tubular cells in primary culture. Amer. Physiol. 246, F757–F764.
Matsuo, S., Fukatsu, A., Taub, M. L., Caldwell, P. R. B., Brentjens, J. R., and Andres, G. (1987) Nephrotoxic glomerulonepritis induced in the rabbit by antiendothelial antibodies. J. Clin. Invest. 79, 1798–1811.
Yang, I. S., Goldinger J. M., Hong, S. K., and Taub, M. (1988) Preparation of basolateral membranes that transport p-aminohippurate from primary cultures of rabbit kidney proximal tubule cells. J. Cell. Physiol. 135, 481–487.
Fine, L. G. and Sakhrani, L. M. (1986) Proximal tubular cells in primary culture. Mineral Elec. Metab. 12, 51–57.
Wang, Y. and Taub, M. (1987) Unpublished observations.
Cook, W. F. and Pickering, G. W. (1958) A rapid method for separating glomeruli from rabbit kidney. Nature 182, 1103,1104.
Taub, M. and Wang, Y. (1987) Control of rabbit kidney proximal tubule cell growth and function by extracellular matrix components, in Biology of Growth Factors, Triennial Symposium, program and abstracts, U. Toronto, Ontario, Canada.
Aleo, M. D., Taub, M. L., Olson, J. R., Nickerson, P. A., and Kostyniak, P. J. (1987) Primary cultures of rabbit renal proximal tubule cells as an in vitro model of nephro-toxicity: effects of 2 mercurials, in In Vitro Toxicology: Approaches to Validation, vol. 5 (Goldberg, Alan M., ed.), Liebert, New York, pp. 211–225.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 The Humana Press Inc.
About this protocol
Cite this protocol
Taub, M. (1990). Primary Kidney Cells. In: Walker, J.M., Pollard, J.W., Walker, J.M. (eds) Animal Cell Culture. Methods in Molecular Biology, vol 5. Humana Press. https://doi.org/10.1385/0-89603-150-0:189
Download citation
DOI: https://doi.org/10.1385/0-89603-150-0:189
Publisher Name: Humana Press
Print ISBN: 978-0-89603-150-0
Online ISBN: 978-1-59259-492-4
eBook Packages: Springer Protocols