Abstract
Cell culture has become an integral part of the daily routine of most oncology laboratories. It has enabled researchers to investigate a wide range of cellular parameters in a defined system in which the experimental conditions can be controlled and repeated. Although the manufacturers of tissue culture materials are continually improving their products, cell attachment and initial survival of primary cultures from tumor cells are still problems in many laboratories. Many different approaches have been taken to circumvent this problem, and coating of tissue culture dishes with attachment enhancers, such as polyamino acids (1), fibronectin (2), laminin (3), and collagen (4), has been found helpful. For a long time, it was known that endothelial cells produce a basement membrane, and this led to the use of bovine corneal basement membrane by Gospodarowicz et al. (5,6), in their research into the phenomenon of regeneration and nonregeneration of corneal endothelium in different species. The application of this bovine corneal extracellular matrix (bECM) has since been greatly expanded (5,6). bECM has found broad approval, and has been used for mammary carcinoma (7), urological tumors (8), and different kinds of pituitary adenomas (9,10) as well as CNS tumors (11).
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Bottenstein, J. E. and Sato, G. H. (1980) Fibronectin and polylysine requirement for proliferation of neuroblastoma cells in defined medium. Exp. Cell Res. 129,361–366.
Terranova, V. P., Aumalley, M., Sultan, L. H., Martin, G. R., and Kleinman, H. K. (1986) Regulation of cell attachment and cell number by fibronectin and laminin. J. Cell. Physiol. 127, 473–479.
Couchman, J. R., Hook, M., Rees, D., and Timpl, R. (1983) Adhesion, growth, and matrix production by fibroblasts on laminin substrates. J. Cell. Biol. 96, 177–183.
Varani, J., Carey, T. E., Fligiel, S. E. G., McKeever, P. E., and Dixit, V. (1987) Tumor type-specific differences in cell-substrate adhesion among human tumor cell lines. Int. J. Cancer 39, 397–403.
Gospodarowicz, D., Vlodavsky, I., and Savion, N. (1981) The role of fibroblast growth factor and the extracellular matrix in the control of proliferation and differentiation of corneal endothelial cells. Vision Res. 21,87–103.
Gospodarowicz, D., Cohen, D., and Fujii, D. K. (1982) Regulation of cell growth by the basal lamina and plasma factors: Relevance to embryonic control of cell proliferation and differentiation, in Cold Spring Harbor Conference on Cell Proliferation Vol. 9 (Cold Spring Harbor Laboratories, Cold Spring Harbor, New York), pp. 95–124.
Wichia, M. S., Lowrie, G., Kohn E., Bagavandoss, P., and Mahn, T. (1982) Extracellular matrix promotes mammary epithelial growth and differentiation in vitro. Proc. Natl. Acad. Sci. 79, 3213–3217.
Pavelic, K., Bulbul, M. A., Slocum, H. K., Pavelic, Z. P., Rustum, Y. M., Niedbala, M. J., and Bernacki, R. J. (1986) Growth of human urological tumors on extracellular Matrix as a model for the in vitro cultivation of primary tumor explants. Cancer Res. 46, 3653–3662.
Westphal, M., Jaquet, P., and Wilson, C. B. (1986) Long-term culture of human corticotropin-secreting adenomas on extracellular matrix and evaluation of serum-free conditions. Ada Neuropath. 71, 142–149.
Westphal, M., Hahn, H., and Lüdecke, D. K. (1987) Culture of dispersed cells from human pituitary adenomas from acromegalic patients on extracellular matrix. In Growth Hormone, Growth Factors, and Akromegaly (Lüdecke, D. K. and Tolis, eds.), Raven Press, New York, pp. 125–133.
Westphal, M., Hänsel, M., Brunken, M., König, A., Köppen, J. A., and Herrman, H. D. (1987) Initiation of primary cell cultures from human intracranial tumors on extracellular matrix from bovine corneal endothelial cells. Exp. Cell. Biol 55, 152–163.
Ill, C. R. and Gospodarowicz, D. (1982) Factors involved in supporting the growth and steroidogenic functions of bovine adrenal cortical cells maintained on extracellular matrix and exposed to a serum-free medium. J. Cell. Physiol. 113, 373–384.
Vlodavsky, I., Lui, G. M., and Gospodarowicz, D. J. (1980) Morphological appearance, growth, behavior, and migratory activity of human tumor cells maintained on extracellular matrix versus plastic. Cell 19, 607–616.
Rutka, J. T. (1986) Effects of extracellular matrix proteins on the growth and differentiation of an anaplastic glioma cell line. Can. J. Neurol. Sci. 13, 301–306.
Weiner, R. I., Bethea, C. L., Jaquet, P., Ramsdell, J. S., and Gospodarowicz, D. J. (1983) Culture of dispersed anterior pituitary cells on extracellular matrix. Methods in Enzymol. 103, 287.
Gospodarowicz, D., Cheng, J., Lui, G. M., Baird, A., and Bühlen, P. (1984) Isolation of brain fibroblast growth factor by heparin sepharose affinity chromatography: Identity with pituitary fibroblast growth factor. Proc. Natl. Acad. Sci. 81, 6963–6967.
Chen, J.-M. and Chen, W. T. (1987) Fibronectin degrading proteases from the membranes of transformed cells. Cell 48, 193–203.
Shapiro, J. R. (1986) Biology of gliomas: Chromosomes, growth factors, and onco-genes. Seminars in Oncology 13,4–15.
Vlodavsky, I., Folkman, J., Sullivan, R., Fridman, R., Ishai-Michaeli, R., Sasse, J., and Klagsbrun, M. (1987) Endothelial cell-derived basic fibroblast growth factor: Synthesis and deposition into subendothelial extracellular matrix. Proc. Natl. Acad. Sci. 84, 2292–2296.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 The Humana Press Inc.
About this protocol
Cite this protocol
Westphal, M., Hänsel, M., Nausch, H., Rohde, E., Herrmann, HD. (1990). Culture of Human Brain Tumors on an Extracellular Matrix Derived from Bovine Corneal Endothelial Cells and Cultured Human Glioma Cells. In: Walker, J.M., Pollard, J.W., Walker, J.M. (eds) Animal Cell Culture. Methods in Molecular Biology, vol 5. Humana Press. https://doi.org/10.1385/0-89603-150-0:113
Download citation
DOI: https://doi.org/10.1385/0-89603-150-0:113
Publisher Name: Humana Press
Print ISBN: 978-0-89603-150-0
Online ISBN: 978-1-59259-492-4
eBook Packages: Springer Protocols