Isoelectric Focusing and Immunofixation of Monoclonal Antibodies

  • Björn Gustafsson
Part of the Methods in Molecular Biology book series (MIMB, volume 5)


Isoelectric focusing of monoclonal antibodies in agarose gel is one of several ways to characterize a hybridoma. Isoelectric focusing is performed on each monoclonal antibody, and the mouse immunoglobulin bands are immobilized by soaking the gel with rabbit antimouse immunoglobulin before staining with Coomassie Blue.


Plastic Film Rubber Roller Coomassie Brilliant Blue Staining Filter Strip Electrode Strip 
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  1. 1.
    Gustafsson, B. and Holme, T. (1983) Monoclonal antibodies against group-and type-specific lipopolysaccharide antigens of Vibrio cholerae O:1. J. Clin. Microbiol. 18, 480–485.PubMedGoogle Scholar

Copyright information

© The Humana Press Inc. 1990

Authors and Affiliations

  • Björn Gustafsson
    • 1
  1. 1.Karolinska Institute and National Bacteriological LaboratoryStockholmSweden

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