Isoelectric Focusing and Immunofixation of Monoclonal Antibodies
Part of the
Methods in Molecular Biology
book series (MIMB, volume 5)
Isoelectric focusing of monoclonal antibodies in agarose gel is one of several ways to characterize a hybridoma. Isoelectric focusing is performed on each monoclonal antibody, and the mouse immunoglobulin bands are immobilized by soaking the gel with rabbit antimouse immunoglobulin before staining with Coomassie Blue.
KeywordsPlastic Film Rubber Roller Coomassie Brilliant Blue Staining Filter Strip Electrode Strip
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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© The Humana Press Inc. 1990