Long-Term B Lymphoid Cultures from Murine Bone Marrow

Establishment and Cloning by Using Stromal Cell Line AC 6.21
  • Cheryl A. Whitlock
  • Christa E. Muller-Sieburg
Part of the Methods in Molecular Biology book series (MIMB, volume 5)


Nearly all hematopoietic cells in mammals derive from precursors that undergo much or all of their development in the bone marrow. In vitro models for many lineages are available and represent modifications of the original bone marrow culture system designed by Dexter and Lajtha (1) and described elsewhere in this book. In this chapter, we describe a second bone marrow culture system, first reported in 1982 (2), that provides an in vitro environment selectively supporting long-term proliferation and differentiation of early B lymphocyte lineage cells. This method can be used to obtain heterogeneous populations of immature precursors of the B cell lineage greatly enriched from other hematopoietic cell types. Clonal populations can also be obtained by extension of this method to limiting dilution culture.


Stromal Cell Lymphoid Cell Feeder Layer Nonadherent Cell Bone Marrow Culture 
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Copyright information

© The Humana Press Inc. 1990

Authors and Affiliations

  • Cheryl A. Whitlock
    • 1
  • Christa E. Muller-Sieburg
    • 2
  1. 1.Stanford University Medical CenterPalo Alto
  2. 2.Lilly Research LaboratoriesLa Jolla

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