Abstract
The successful measurement of cerebral high-energy phosphates (ATP, ADP, AMP, and phosphocreatine) by the enzymatic techniques to be described in this chapter is dependent on: (1) adequate tissue fixation, (2) preparation, dissection, and weighing of samples to be analyzed, and (3) fluorometric enzymatic analysis of the sample for high-energy phosphates. The fact that the brain is organized in a highly complex fashion necessitates the measurement of metabolites in very small pieces of tissue, down to and including single cells. Therefore, the methodology for dissection, weighing, and analysis of tissue varies to some extent with the size of the sample.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Barbehenn E. K., Noelker D. M., Chader G. J., and Passonneau J. V. (1985) Adenine nucleotide and P-creatine levels in layers of frog retina as a function of dark and light adaption. Exp. Eye Res. 40, 675–686.
Barbehenn E. K., Law M. M., Brown J. G., and Lowry O. H. (1976) Measurement of 5′-adenylic acid by stimulation of phosphorylase a. Anal. Biochem. 70, 554–562.
Lowry O. H. and Passonneau J. V. (1972) A Flexible System of Enzymatic Analysis Academic, New York.
Medina M. A., Deam A. P., and Stavinoha W. B. (198) Inactivation of Brain Tissue by Microwave Irridation, in Cerebral Energy Metabolism and Neural Function (Passonneau J. V., Hawkins R. A., Lust W. D., and Welsh F. A., eds.) Williams and Wilkins, Baltimore.
Veech R. L., Harris R. L., VeLoso D., and Veech E. H. (1973) Freeze-blowing: A new technique for the study of brain in vivo. J. Neurochem. 20, 183–187.
Welsh F. A. (1980) In situ Freezing of Cat Brain, in Cerebral Energy Metabolism and Neural Function (Passonneau J V, Hawkins R. A., Lust W. D., and Welsh F. A., eds.) Williams and Wilkins, Baltimore.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 The Humana Press Inc
About this protocol
Cite this protocol
Dworsky, S., Namovic, M., McCandless, D.W. (1989). High-Energy Phosphates in Brain from Dissected Freeze-Dried Regions to Single Cells. In: Boulton, A.A., Baker, G.B., Butterworth, R.F. (eds) Carbohydrates and Energy Metabolism. Neuromethods, vol 11. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-143-8:343
Download citation
DOI: https://doi.org/10.1385/0-89603-143-8:343
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-143-2
Online ISBN: 978-1-59259-616-4
eBook Packages: Springer Protocols