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Use of Elutips to Purify DNA

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New Nucleic Acid Techniques

Part of the book series: Methods in Molecular Biology ((MIMB,volume 4))

Abstract

Elutips are essentially minicolumns of reverse-phase resin that offer an extremely rapid method for DNA purification and may often replace conventional techniques for manipulation of DNA. The Elutip-d columns commercially available are manufactured by Schleicher and Schuell. Each column consists of a pipet tip, such as may be used on a 0–200 µL automatic pipet, that is filled with an insoluble matrix.

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References

  1. Maxam, A. M. and Gilbert, W. (1980) Sequencing end-labeled DNA with base-specific chemical cleavages. Meth. Enzymol. 65, 499–560.

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  2. Zarbl, H., Sukumar, S., Arthur, A.V., Martin-Zanca, D., and Barbacid, M. (1985) Direct mutagenesis of Ha-ras-1 oncogenes by N-nitroso-N-methylurea during initiation of mammary carcinogenesis in rats. Nature 315, 382–385.

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  3. Schmitt, J. J. and Cohen, B. N. (1983) Quantitative isolation of DNA restriction fragments from low-melting agarose by Elutip-D affinity chromatography. Anal. Biochem. 133, 462–464.

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© 1988 The Humana Press Inc.

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Van Helden, P.D. (1988). Use of Elutips to Purify DNA. In: Walker, J.M. (eds) New Nucleic Acid Techniques. Methods in Molecular Biology, vol 4. Humana Press. https://doi.org/10.1385/0-89603-127-6:61

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  • DOI: https://doi.org/10.1385/0-89603-127-6:61

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-127-2

  • Online ISBN: 978-1-59259-491-7

  • eBook Packages: Springer Protocols

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