Abstract
In wheat, growth is from a basal meristem: The cells of the young shoot form a developmental array from the tip (oldest) to the base (youngest) of the leaf. In cells from a specific part of the leaf (and so of a certain age), chloroplast DNA is found to be replicating. In seedlings up to 10 d old, chloroplast DNA replication is found in the region from 1 to 4 cm from the leaf base (1) If chloroplasts are isolated from this region of leaf and incubated in the presence of a radioactively labeled DNA precursor, radioactively labeled DNA can be produced in vitro.
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References
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© 1988 The Humana Press Inc.
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Jones, M.C. (1988). DNA Labeling by Isolated Wheat Chloroplasts. In: Walker, J.M. (eds) New Nucleic Acid Techniques. Methods in Molecular Biology, vol 4. Humana Press. https://doi.org/10.1385/0-89603-127-6:545
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DOI: https://doi.org/10.1385/0-89603-127-6:545
Publisher Name: Humana Press
Print ISBN: 978-0-89603-127-2
Online ISBN: 978-1-59259-491-7
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