Skip to main content
SpringerLink
Log in

Isolation of Wheat Chloroplasts and Chloroplast DNA

  • Protocol
New Nucleic Acid Techniques

Part of the book series: Methods in Molecular Biology ((MIMB,volume 4))

  • 3605 Accesses

Abstract

Chloroplasts in common with other plastids and mitochondria have their own nucleic material, in the form of multiple copies of a circular DNA molecule, normally of 120–160 kbp.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Wells, R. and Ingle, J. (1970) The constancy of the buoyant density of chloroplast and mitochondrial deoxyribonucleic acid in a range of higher plants. Plant Physiol. 46, 178–179.

    Article  PubMed  CAS  Google Scholar 

  2. Vedel, F. and Quetier, F. (1978) Hydrolyse specifique de l’ADN chloroplastique et l’ADN mitochondrial des vegetaux superieurs par les enzymes de restriction. Physiol. Veg. 16, 411–425.

    CAS  Google Scholar 

  3. Kolodner, R. and Tewari, K.K. (1975) The molecular size and conformation of chloroplast DNA from higher plants. Biochem. Biophys. Acta 402, 372–390.

    PubMed  CAS  Google Scholar 

  4. Jones, M.C. and Boffey, S.A (1984) Deoxyribonuclease activities of wheat seedlings. FEBS Lett. 174, 215–218.

    Article  CAS  Google Scholar 

  5. Leegood, R.C. and Walker, D.A. (1979) Isolation of protoplasts and chloroplasts from flag leaves of Triticum aestivum L. Plant Physiol. 63, 1212–1214.

    Article  PubMed  CAS  Google Scholar 

  6. Grierson, D. and Covey, S. (1976) The properties and function of rapidly-labelled nuclear DNA. Planta 130, 317–321.

    Article  CAS  Google Scholar 

  7. Bowman, C.M. and Dyer, T.A. (1982) Purification and analysis of DNA from wheat chloroplasts isolated in non-aqueous media. Anal Biochem. 122, 108–118.

    Article  PubMed  CAS  Google Scholar 

  8. Rhodes, P.R. and Kung, S.D. (1981) Chloroplast deoxyribonucleic acid isolation: Purity achieved without nuclease digestion. Can. J. Biochem. 59, 911–915.

    PubMed  CAS  Google Scholar 

  9. Terri, T., Saura, A., and Lokki, J. (1984) Chloroplast DNA from Calmagrostis species by selective lysis of organelles. Hereditas 101, 123–126.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. The Biotechnology Centre, University of Cambridge, Cambridge, UK

    Matthew Clement Jones

Authors
  1. Matthew Clement Jones
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. The Hatfield Polytechnic, Hatfield, Hertfordshire, UK

    John M. Walker

Rights and permissions

Reprints and permissions

Copyright information

© 1988 The Humana Press Inc.

About this protocol

Cite this protocol

Jones, M.C. (1988). Isolation of Wheat Chloroplasts and Chloroplast DNA. In: Walker, J.M. (eds) New Nucleic Acid Techniques. Methods in Molecular Biology, vol 4. Humana Press. https://doi.org/10.1385/0-89603-127-6:537

Download citation

  • DOI: https://doi.org/10.1385/0-89603-127-6:537

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-127-2

  • Online ISBN: 978-1-59259-491-7

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation