Abstract
The process whereby part of the energy evolved in a chemical reaction is emitted as light is known as luminescence. When luminol is oxidized by hydrogen peroxide in the presence of horseradish peroxidase (HRP), light is evolved (1). This is shown in Fig. 1. Luciferin not only enhances the reaction, but prolongs the emission over several minutes (2). The light may be measured in a luminometer or visualized as the blackening of photographic film, as will be described.
Oxidation of luminol in the presence of hydrogen peroxide and HRP (the catalyst).
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References
Auses, J.P., Cook, S.L., and Maloy, J.T. (1975) Chemiluminescent enzyme method for glucose. Anal. Chem. 47, 244–249.
Whitehead, T.P., Thorpe, G.H.G., Carber, T.J.N., Groneutt, C., and Kricka, L.J. (1983) Enhanced luminescent procedure for sensitive determination of peroxidase-labelled conjugate in immunoassay. Nature 305, 158–159.
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© 1988 The Humana Press Inc.
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Woodhead, J.L., Figueiredo, H., Malcolm, A.D.B. (1988). Luminescent Detection of Specific DNA Sequences. In: Walker, J.M. (eds) New Nucleic Acid Techniques. Methods in Molecular Biology, vol 4. Humana Press. https://doi.org/10.1385/0-89603-127-6:421
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DOI: https://doi.org/10.1385/0-89603-127-6:421
Publisher Name: Humana Press
Print ISBN: 978-0-89603-127-2
Online ISBN: 978-1-59259-491-7
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