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New Nucleic Acid Techniques pp 27–38Cite as

Hybrid-Release Translation

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 4))

Abstract

Many situations arise in recombinant DNA research in which it is necessary to identify the mRNA encoded by a particular cloned DNA sequence. Cloned DNA fragments can be characterized by hybridization to mRNA, the complementary mRNA then being identified by translation in vitro. There are two different approaches to this procedure; hybrid-arrest translation (1) and hybrid-release translation (2). In the former, hybridization of cloned DNA to an mRNA population in solution can be used to identify the complementary mRNA, since the mRNA-DNA hybrid will not be translated in vitro (see Chapter 4). In the latter, described here, cloned DNA bound to a solid support is used to isolate the complementary mRNA, which can then be eluted and translated in vitro.

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Author information

Authors and Affiliations

  1. School of Life Sciences, Leicester Polytechnic, Leicester, UK

    Adrian Slater

Authors
  1. Adrian Slater
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Editor information

Editors and Affiliations

  1. The Hatfield Polytechnic, Hatfield, Hertfordshire, UK

    John M. Walker

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© 1988 The Humana Press Inc.

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Slater, A. (1988). Hybrid-Release Translation. In: Walker, J.M. (eds) New Nucleic Acid Techniques. Methods in Molecular Biology, vol 4. Humana Press. https://doi.org/10.1385/0-89603-127-6:27

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  • DOI: https://doi.org/10.1385/0-89603-127-6:27

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-127-2

  • Online ISBN: 978-1-59259-491-7

  • eBook Packages: Springer Protocols

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