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Immunoscreening of λgt11 Expression Libraries Using an Avidin–Biotin Detection System

  • Robert McGookin
Part of the Methods in Molecular Biology book series (MIMB, volume 4)

Abstract

Although the avidin-biotin detection system has been used for immunochemical staining of tissues for some time, its use for amplification in immunoassays was not described until 1979 (1). The biological basis for the system is the very tight binding of four molecules of biotin to one of avidin. This has an amplification ability that can be used to increase the sensitivity of detection of antibodies or other biotin-labeled molecules. Using antibodies raised to a purified peptide and an efficient expression system, such as λgtll (2), the clone(s) containing the cDNA for that peptide can usually be identified by detection of expressed antigens.

Keywords

Tight Binding Nitrocellulose Filter Primary Antiserum Immunochemical Staining Maximum Amplification 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Guesdon, J.I., Ternynck, T., and Avrameas, S. (1979) The use of avidin-biotin interaction for immunoenzymatic techniques. J. Histochem. Cytochem. 27, 1131–1139.PubMedGoogle Scholar
  2. 2.
    Huynh, T.V., Young, R.A., Davis, R.W. (1985) Constructing and Screening cDNA Libraries in Lambda gt10 and Lambda gt11, in DNA Cloning, A Practical Approach (Glover, D.M., ed.) Vol. 1, IRL Press, Oxford and Washington, DC.Google Scholar
  3. 3.
    Buckland, R.M. (1986) Strong signals from streptavidin-biotin. Nature 320, 557–558.CrossRefGoogle Scholar

Copyright information

© The Humana Press Inc. 1988

Authors and Affiliations

  • Robert McGookin
    • 1
  1. 1.Inveresk Research International Ltd.MusselburghScotland

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