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Colloidal Gold for the Detection of Proteins on Blots and Immunoblots

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 3))

Abstract

Immunoblotting techniques, in which electrophoretically separated proteins are transferred to the surface of nitrocellulose or nylon membranes (“Western” blots), are becoming increasingly popular in the analysis of protein systems. Transfer techniques in protein blotting have been covered in this series (Chapter 20 in vol. 1, and Chapters 28 and 29 in this volume). In this chapter we will describe the use of the colloidal gold marking system for the detection of proteins on blots.

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© 1988 The Humana Press Inc.

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Jones, A., Moeremans, M. (1988). Colloidal Gold for the Detection of Proteins on Blots and Immunoblots. In: Walker, J.M. (eds) New Protein Techniques. Methods in Molecular Biology™, vol 3. Humana Press. https://doi.org/10.1385/0-89603-126-8:441

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  • DOI: https://doi.org/10.1385/0-89603-126-8:441

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-126-5

  • Online ISBN: 978-1-59259-490-0

  • eBook Packages: Springer Protocols

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