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Two-Dimensional (Crossed) Immunoelectrophoresis

  • Protocol
New Protein Techniques

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 3))

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Abstract

Two-dimensional (2-D) immunoelectrophoresis, also known as crossed immunoelectrophoresis, is a particularly useful technique for the quantitation of mixtures of proteins and the analysis of the composition of protein mixtures. The method consists of two sequential electrophoretic steps:

  1. 1.

    The first dimension, during which the protein mixture to be analyzed is separated by electrophoresis in an agarose gel.

  2. 2.

    The second dimension, during which the separated proteins are electrophoresed at right angles into a freshly applied layer of agarose containing a predetermined amount of antibody.

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Further Reading

  1. Axelsen, N.H., Kroll, J., and Week, B., eds. (1973) A Manual of Quantitative Immunoelectrophoresis—Methods and Applications Universitatsforgleget, Oslo.

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  2. Axelsen, N.H., ed. (1983) Handbook of Immunoprecipitation-in-Gel Techniques Blackwell Scientific, Oxford, London.

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Author information

Authors and Affiliations

  1. Biological Sciences, The Hatfield Polytechnic, Hatfield, Hertfordshire, UK

    John M. Walker

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  1. John M. Walker
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Editors and Affiliations

  1. The Hatfield Polytechnic, Hatfield, Hertfordshire, UK

    John M. Walker

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© 1988 The Humana Press Inc.

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Walker, J.M. (1988). Two-Dimensional (Crossed) Immunoelectrophoresis. In: Walker, J.M. (eds) New Protein Techniques. Methods in Molecular Biology™, vol 3. Humana Press. https://doi.org/10.1385/0-89603-126-8:299

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  • DOI: https://doi.org/10.1385/0-89603-126-8:299

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-126-5

  • Online ISBN: 978-1-59259-490-0

  • eBook Packages: Springer Protocols

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