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Isolation, Separation, and Analysis of Phosphoinositides from Biological Sources

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Lipids and Related Compounds

Part of the book series: Neuromethods ((NM,volume 7))

Abstract

The phosphoinositides, namely phosphatidylinositol (PI), phosphatidylinositol 4-phosphate (PIP), and phosphatidylinositol 4,5-bisphosphate (PIP2), are acidic lipids that are present in all animal tissues. The markedly polar character of these lipids makes conventional lipid extraction and assay procedures unsuitable for their analysis. Hence, specific analytical procedures have been devised in a number of laboratories for the quantitative extraction, separation, and assay of this class of lipids. These methods are reviewed, and details of analytical procedures used in our laboratory are given below.

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Author information

Authors and Affiliations

  1. University of Michigan, Neuroscience Laboratory, Ann Arbor, Michigan

    Amiya K. Hajra, Stephen K. Fisher & Bernard W. Agranoff

Authors
  1. Amiya K. Hajra
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  2. Stephen K. Fisher
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  3. Bernard W. Agranoff
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Editor information

Editors and Affiliations

  1. University of Saskatchewan, Saskatoon, Canada

    Alan A. Boulton

  2. University of Alberta, Edmonton, Canada

    Glen B. Baker

  3. The Ohio State University, Columbus, Ohio

    Lloyd A. Horrocks

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© 1988 The Humana Press Inc.

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Hajra, A.K., Fisher, S.K., Agranoff, B.W. (1988). Isolation, Separation, and Analysis of Phosphoinositides from Biological Sources. In: Boulton, A.A., Baker, G.B., Horrocks, L.A. (eds) Lipids and Related Compounds. Neuromethods, vol 7. Humana Press. https://doi.org/10.1385/0-89603-124-1:211

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  • DOI: https://doi.org/10.1385/0-89603-124-1:211

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-124-1

  • Online ISBN: 978-1-59259-612-6

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