Abstract
Nuclear magnetic resonance (NMR) methods applicable to the study of functioning brainin situ are new in neuroscience, and many neuroscientrsts may not be familiar with them. Although we have provrded some explanatory material in this review, many readers may wish to consult other works (Gadian, 1982; Moore, 1984) which provide access to a wider range of basic NMR literature, as well as mtroductions to NMR theory for scientists in other disciplmes. For an excellent discussion of in vivo NMR studies of muscle, which are not covered here, the reader is referred to a recent review (Radda et al, 1984).
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References
Ackerman J J H., Grove T. H., Wong G. G., Gadian D G., and Radda G. K (1980) Mapping of metabolites in whole animals by 31P NMR using surface coilsNature (Lond)283, 167–170.
Ackerman J. J. H, Berkowitz B. A., and Deuel R K Phosphorus-31 NMR of rat brain in vivo with bloodless perfluorocarbon perfused rat.Biochem Biophys Res Commun. 119, 913–919.
Ackerman J. J. H, Evelhoch J L., Berkowitz B. A., Kichura G. M., Deuel R. K., and Lown K. S. (1984b) Selective suppression of the cranial bone resonance from 31P NMR experiments with rat brain in vivo.J. Magn. Reson 56, 318–322.
Alger J. R and Shulman R. G. (1984) NMR studies of enzymatic rates in vitro and in viva by magnetization transfer.Quart Rev Biophys. 17, 83–124
Andrew E. R. (1984) A historical review of NMR and its clinical applications.Br. Med Bull. 40, 115–119
Aue W. P., Muller S., Cross T. A., and Seelig J. (1984) Volume-selective excitation. A novel approach to topical NMR.J. Magn. Reson. 56, 350–354.
Balaban R. S., Kantor H. L, and Ferretti J. A. (1983) In viva flux between phosphocreatine and adenosine triphosphate determined by two dimensional phosphorus NMR.J. Biol. Chem. 258, 12787–12789.
Behar K. L., den Hollander J. A., Petroff O A C., Hetherington H, Prichard J. W, and Shulman R. G. (1985) The effect of hypoglycemic encephalopathy upon amino acids, high energy phosphates, and pHi in the rat brain in viva. Detection by sequential1H and31P NMR spectroscopy.J Neurochem 44, 1045–1055
Behar K. L., den Hollander J. A., Stromski M. E, Ogino T., Shulman R. G., Petroff O. A. C., and Prichard J. W. (1983) High-resolution1H nuclear magnetic resonance study of cerebral hypoxia in viva.Proc. Natl. Acad. Ser. USA 80, 4945–4948.
Behar K. L., Petroff O C, Prichard J. W., Alger J. R., and Shulman R. G. (1986) Detection of metabolltes in rabbit brain by13C-NMR spectroscopy following administration of [l−13C] glucose.Magn. Res Med 3, 911–920
Behar K L, Rothman D L, Shulman R. G, Petroff O. A. C., and Prichard J. W. (1984) Detection of cerebral lactate in viva during hypoxemia by1H NMR at relatively low field strengths (1.9 Tesla).Proc. Natl. Acad. Sci. USA 81, 2517–2519.
Bendall M. R. and Gordon R E. (1983) Depth and refocusing pulses designed for multipulse NMR with surface coils.J. Magn. Reson. 53, 365–385.
Bloch F, Hansen W. W., and Packard M. E. (1946) Nuclear induction.Phys. Rev. 69, 127.
Bottomley P. A., Edelstein W. A., Foster T. H., and Adams W. A. (1985) In vivo solvent suppressed localized hydrogen nuclear magnetic resonance (NMR). A new window to metabolism?.Proc. Natl Acad Sci. USA 82, 2148–2152.
Bottomley P A., Hart H. R., Edelstein W. A., Schenk J. F., Smith L. S., Leue W M, Mueller O. M, and Reddington R W. (1984) Anatomy and metabolism of the normal human brain studied by magnetic resonance at 1.5 Tesla.Radiology 150, 441–446.
Bottomley P. A, Kogure K, Namon R., and Alonso O. F. (1982) Cerebral energy metabolism in rats studred by phosphorus nuclear magnetic resonance using surface coilsMap. Reson Imug. 1, 81–85.
Brooks D. J., Lammertsma A. A, Beaney R P., Leenders K. L., Buckingham P. D., Marshall J, and Jones T. (1984) Measurement of regional cerebral pH in human subjects using continuous inhalation of llCO2 and positron emission tomography.J. Cereb. Blood Flow Metab 4, 458–465.
Budinger T F. and Cullander C. (1983) Biophysical Phenomena and Health Hazards of In Viva Magnetic Resonance, inClinical Magnetic Resonance Imaging (Margulis A. R, Higgins C, Kaufman L, and Crooks L eds) Radiol Res. Educ. Foundation, San Francisco.
Bydder G. M. (1984) Nuclear magnetic resonance imaging of the brainBr. Med Bull 40, 170–174.
Cady E., Costello A. M, Dawson M. J, Delpy D. T, Hope P. L, Reynolds E O. R., Tofts P S, and Wilkie D R. (1983) Noninvasive investigation of cerebral metabolism in newborn infants by phosphorus nuclear magnetic resonance spectroscopy.Luncet ii, 1059–1062
Chance Nakase Y, Bond M, Leigh J S., and McDonald G. (1978) Detection of 31P nuclear magnetic resonance signals in brain by in viva and freeze-trapped assays.Proc. Natl. Acad. Sci USA 75, 4925–4929.
Decorps M., Lebas J. L, Leviel J L, Confort S, Remy C., and Benabid A L. 1984. Analysis of brain metabolism changes induced by acute potassium cyanide intoxication by 31P NMR in vivo using chronically implanted surface coilsFEBS Lett. 168, 1–6
Delpy D T., Gordon R E., Hope P. L, Parker D, Reynolds E O. R., Shaw D, and Whitehead M. D (1982) Nonmvasive investigation of cerebral ischemia by phosphorus nuclear magnetic resonancePediatrics 70, 310–313.
Gadian D. G (1982)Nuclear Magnetic Resonance and its Applications to Living Systems Clarendon Press, Oxford
Glonek T., Kopp S. J., Kot E., Pettegrew J. W., Harrison W. H., and Cohen M. M (1982) P-31 nuclear magnetic resonance analysis of brain: The perchloric acid extract spectrumJ Neurochem 39, 1210–1219.
Hetherington H. P., Avison M J., and Shulman R G. (1985)1H homonuclear editing of rat brain using semi-selective pulses.Proc Natl. Acad. Sci. USA 82, 3115–3118.
Hilberman M., Subramanian V. H., Haselgrove J., Cone J. B, Egan J W., Gyulai L, and Chance B. (1984) In viva time-resolved brain phosphorus nuclear magnetic resonance.J Cereb. Blood Flow Metab 4, 334–342
Hope P L, Cady E B, Tofts P S., Hamilton P A., Costello A M., Delpy D T., Chu A., and Reynolds E. O. R. (1984) Cerebral energy metabolism studied with phosphorus NMR spectroscopy in normal and birth-asphyxiated infantsLancet ii, 366–370
Hore P J. (1983) Solvent suppression in fourier transform nuclear magnetic resonanceJ Magn. Reson. 55, 283–300
Matthews P M., Bland J L, Gadian D. G, and Radda G. K. (1982) A 31P-NMR saturation transfer study of the regulation of creatine kinase in the rat heartBiochem. Biophys Acta 721, 312–320.
Maudsley A A and Hilal S K (1984) Biological aspects of sodium-23 imagingBr. Med Bull. 40, 165–166.
Maudsley A. A, Hilal S K, Simon H E., and Wittekoek S (1984) In viva MR spectroscopic imaging with P-31Radiology 153, 745–750
Moore W S (1984) Basic physics and relaxation mechanismsBr. Med Bull. 40, 120–124.
Morris G A and Freeman R (1978) Selective excitation in Fourier transform nuclear magnetic resonance.J Magn Reson 29, 433–462
Naruse S, Horikawa Y, Tanaka C, Hirakawa K., Nishikawa H, and Watari H. (1984) In viva measurement of energy metabolism and the concomitant monitoring of encephalogram in experimental cerebral ischemia.Brain Res 206, 370–372.
Naruse S, Takada S, Koizuka I, and Watari H. (1983) In viva31P NMR studies on experimental cerebral infarction.Japan J. Physlol 33, 19–28
NRPB (1983) Revised guidance on acceptable limits of exposure during nuclear magnetic resonance clinical imaging (Statement by National Radiological Protection Board).Br. J Radiol 56, 974–977.
Petroff O A C. and Prichard J W. (1983) Cerebral pH by NMRLancet ii, 105–106.
Petroff O A C, Prichard J W., Behar K L., Alger J R, den Hollander J. A, and Shulman R G. (1985) Cerebral intracellular pH by31P nuclear magnetic resonance spectroscopyNeurology 35, 781–788.
Petroff O C, Prichard J W, Behar K. L, Alger J. R., and Shulman R G (1984) In viva phosphorus nuclear magnetic resonance spectroscopy in status epilepticusAnn Neurol. 16, 169–177
Petroff O. A C., Prichard, J. W., Ogina T., Avison M., Alger J R., and Shulman R G (1986)Combined1H and31P magnetic resonance spectroscopy studies of bicuculline-induced seizure in vivo.Ann. Neural 20, 185–193
Prichard J. W, Alger J. R., Behar K L., Petroff O. A. C., and Shulman R. G. (1983) Cerebral metabolic studies in vivo by31P NMR.Proc Natl Acad. Sci. USA 80, 2748–2751
Purcell E. M, Torrey H C, and Pound R. V (1946) Resonance absorption by nuclear magnetic moments in a solid.Phys Rev 69, 37–38.
Radda G. K., Bore P J., and Rajagopalan B. (1984) Cluucal aspects of31P NMR spectroscopy.Br. Med Bull 40, 155–159
Rothman D. L., Behar K. L., Hetherington H. P, and Shulman R G (1984) Homonuclear1H double resonance difference spectroscopy of the rat brain in vivoProc. Natl Acad Sci USA 81, 6330–6334.
Rothman D L, Behar K L., Hetherington H P., den Hollander J. A., Bendall M R, Petroff O C, and Shulman R. G (1985)1H observed13C decoupled spectroscopic measurements of lactate and glutamate in the rat brain in vivo.Proc. Natl. Acad Sci. USA 82, 1633–1637.
Rottenberg D. A, Gmos J. Z., Kearfort K J, Junck L., and Bigner D D (1984) In vivo measurement of regional brain tissue pH using positron emission tomographyAnn. Neural 15(suppl), S98–S102.
Saunders R. D. and Smith H (1984) Safety aspects of NMR clinical imaging.Br. Med. Bull 40, 148–154.
Shoubridge E A, Briggs R W, and Radda G (1982)31P NMR saturation transfer measurements of the steady state rates of creatine kinase and ATP synthetase in the rat brain.FEBS Lett. 140, 288–292
Siesjo B. K. and Agardh C. D. (1983) Hypoglycemia, inHandbook of Neurochemistry, 2nd Ed. (Laltha A, ed.) Plenum, New York.
Sillerud L. O. and Shulman R. G. (1983) High-resolution13C nuclear magnetic resonance studies of glucose metabolism inEscherichia colt. Biochemistry 22, 1087–1094.
Sillerud L. O., Alger J. R., and Shulman R. G. (1981) High-resolution proton NMR studies of intracellular metabolites in yeast using13C decoupling.J Magn Reson 45, 142–150
Syrota A., Castaing M., Rougement D., Berridge M., Baron J. C., Bousser M. G., and Pocidalo J. J (1983) Tissue acid-base balance and oxygen metabolism in human cerebral infarction studied with positron emission tomography.Ann. Neural 14, 419–428
Thulborn K. R, duBoulay G H, Duchen L. W, and Radda G. (1982) A31P nuclear magnetic resonance in vivo study of cerebral ischemia in the gerbil.J. Cereb. Blood Flow Metab. 2, 299–306
Ugurbil K., Maidan R. R., Petein M., Michurski S P., Cohn J. N., and From A. H L (1984) NMR measurements of myocardial CK rates by multiple saturation transferCirculation 70(suppl.), 11–84.
Veech R. L, Lawson J W. R, Cornell N. W., and Krebs H A. (1979) Cytosolic phosphorylation potentialJ iol Chem. 254, 6538–6547.
Younkin D P., Delivoria-Papadopoulos M., Leonard J C., Subramanian V H., Eleff S., Leigh J. S, and Chance B. (1984) Unique aspects of human newborn cerebral metabolism evaluated with phosphorus nuclear magnetic resonance spectroscopyAnn. Neurol. 16, 581–586
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Prichard, J.W., Shulman, R.G. (1988). NMR Spectroscopy of Brain Metabolism In Vivo. In: Boulton, A.A., Baker, G.B., Boisvert, D.P.J. (eds) Imaging and Correlative Physicochemical Techniques. Neuromethods, vol 8. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-116-0:233
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DOI: https://doi.org/10.1385/0-89603-116-0:233
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