Abstract
The discovery that many peptide hormones previously identified in endocrine glands and/or in the endocrine brain (Hökfelt et al., 1980) are also present in central and peripheral neurons has unexpectedly increased the list of potential messengers participating in neuronal communication. To this list we must add other newly discovered peptides first detected as endogenous ligands of brain receptors (Hughes et al., 1975) and peptides whose existence was initially deduced from nucleotide or amino acid sequences in genes or protein precursors studied with the powerful techniques of modern molecular biology (Nakanishi et al., 1979; Lenoir et al., 1985). In order to understand the role of this growing list of peptides in neurohumoral coding of brain function, we must not only study the ways in which they interact with classical transmitter systems and modify the behavior of their target nerve cells, but also the ways in which they are produced, stored, and released.
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Bayón A. and Drucker-Colín R., eds. (1985a) In Vivo Perfusion and Release of Neuroactive Substances: Methods and Strutegies. Academic, Orlando.
Bayón A. and Drucker-Colín R. (1985b), Methodological Alternatives and Experimental Strategies for Studying the In Vivo Perfusion and Release of Neuroactive Substances in the Central Nervous System, in In Vivo Perfusion and Release of Neuroactive Substances (Bayón A. and Drucker-Colín R., eds ), Academic, Orlando.
Bayón A., Rossier J., Mauss A., Bloom F. E., Iversen L. L., and Guillemin R. (1978) In vitro release of methionine-enkephalin and leucine-enkephalin from the rat globus pallidus Proc Natl Acad Sci USA 75, 3503–3506.
Bayón A., Shoemaker W. J., Lugo L, Azad R., Ling N., Drucker-Colín R., and Bloom F E. (1981a) In vivo release of enkephalin from the globus pallidus. Neurosci Lett. 24, 65–70.
Bayón A, Drucker-Colín R, Lugo L, Shoemaker W J, Azad R., Ling N, and Bloom F. E (1981b) Enkephalin Release From the Globus Pallidus. In Vitro and In Vivo Studies, in Regulatory Mechanisms of Synaptic Transmission (Tapia R. and Cotman C. W, eds.), Plenum, New York.
Bayón A., Shoemaker W. J., McGinty J. F., and Bloom F. E. (1983) Immunodetection of Endorphins and Enkephalins: A Search for Reliability, in International Review of Neurobiology vol. 24, Academic, New York.
Bayón A., Solano S., Anton B., Castaño I., and Diaz D. (1985) Push-Pull Perfusion Studies on the In Vivo Release of Proteins, Enzymes, and the Neuroactive Peptides—Enkephalins—From the Rat Brain, in In Vivo Perfusion and Release of Neuroactive Substances (Bayón A and Drucker-Colín R, eds.), Academic, Orlando.
Bennett G. W., Brazell M. P., and Marsden C. A. (1981) Electrochemistry of neuropeptides. A possible method for assay and in viva detection. Life Sci. 29, 1001–1007.
Bourgoin S, Cesselin F., Artaud F., Glowinski J, and Hamon M. (1982) In vivo modulation by GABA-related drugs of Met-enkephalin release in basal ganglia of the cat brain. Brain Res 248, 321–330.
Cesselin F., Soubrie P, Bourgoin S., Artaud F, Reisine T. D., Michelot R., Glowinski J., and Hamon M (1981) In vivo release of Met-enkephalin in the cat brain. Neurosci 6, 301–313.
Delgado J M., Lerma J., Martin del Rio R., and Solis J. M. (1985) Use of Dialytrodes in Brain Neurochemistry, in In Vivo Perfusion and Release of Neuroactive Substances (Bayón A and Drucker-Colín R, eds.), Academic, Orlando.
Dreifuss J J., Mathison R D., and Gáhwiler B. H. (1980) Secretion of Neurohypophysial Peptides, in The Role of Peptides in Neuronal Function (Barker J. L and Smith, Jr., T. G., eds.), Marcel Dekker, New York.
Drouva S. V, Epelbaum J., Héry M., Tapia-Arancibia L., Laplante E., and Kordon C. (1981) Ionic channels involved in the LHRH and SRIF release from rat mediobasal hypothalamus Neuroendocrinology 32, 155–162.
Franco-Bourland R. and Fernstrom J. D. (1981) In vivo biosynthesis of L-(35S)Cys-arginine vasopressin,-oxytocin and-somatostatin: Rapid estimation using reversed phase high pressure liquid chromatography. Endocrinology 109, 1097–1106.
Gaddum J. H. (1961) Push-pull cannulae J. Physiol. 155, 1–2P.
Glowinski J (1981) In vivo release of transmitters in the cat basal ganglia Fed Proc 40, 135–141.
Gray E. G. and Whittaker V. P (1962) The isolation of nerve endings from brain. An electron microscopic study of the cell fragments derived by homogenization and centrifugation. J Anat 96, 79–88.
Greenfield S. A and Shaw S. G. (1982) Release of acetylcholinesterase and aminopeptidase in vivo followmg infusion of amphetamine into the substantia nigra Neuroscience 7, 2883–2893.
Greenfield S., Cheramy A., and Glowmski J. (1983) Evoked release of proteins from central neurons in viva. J Neurochem 40, 1048–1057.
Hajos F. (1975) An improved method for the preparation of synaptosomal fractions in high purity. Brain Res. 136, 387–392.
Hamberger A., Berthold C H., Jacobson I., Karlsson B., Lehmann A., Nystrom B., and Sandberg M. (1985) In Vivo Brain Dialysis of Extracellular Nontransmitter and Putative Transmitter Amino acids, in In Vivo Perfusion and Release of Neuroactive Substances (Bayón A. and Drucker-Colín R., eds ), Academic, Orlando.
Henderson G., Hughes J., and Kosterlitz H W. (1978) In vitro release of Leu-and Met-enkephalin from the corpus striatum. Nature 271, 677–679.
Hokfelt T., Johansson O., Ljungdahl A., Lundberg J and Schultzberg M. (1980) Peptidergic neurones. Nature 284, 515–521.
Honchar M. P., Hartman B. K, and Sharpe L. G (1979) Evaluation of in vivo brain site perfusion with the push-pull cannula. Am. J Physiol 236, R45–56.
Hughes J., Smith T. W, Kosterlitz H. W, Fothergill L H., Morgan B. A., and Morris H. (1975) Identification of two related pentapeptides from the brain with potent opiate agonist activity. Nature 255, 577–579.
Iversen L. L., Iversen S. D., Bloom F. E., Vargo T., and Guillemin R. (1978a) Release of enkephalin from rat globus pallidus in vitro. Nature 271, 679–681.
Iversen L. L., Iversen S D., Bloom F. E, Douglas C., Brown M., and Vale W. (1978b) Calcium-dependent release of somatostatin and neurotensin from rat brain in vitro. Nature 273, 161–163.
Jessell T. M. (1978) Substance P release from the rat substantia nigra. Brain Res 151, 469–478.
Jones, C. A. and Marchbanks R M. (1983) The synthesis and release of [3H-tyrosine1]methionine5-enkephalin from guinea pig brain slices J. Neurochem 40, 357–363.
Klaff L. J., Hudson A. M., Paul M., and Millar R. P (1982) A method for studying synaptosomal release of neurotransmitter candidates as evaluated by studies on cortical cholecystokinin octapeptide release. Peptides 1, 155–161.
Kordon C, Enjalbert A, Hery M., Joseph-Bravo P, Rotsztejn W., and Ruberg M. (1980) Role of Neurotransmitters in the Control of Adenohypophyseal Secretion, in Handbook of the Hypothalamus vol. 2 (Morgane P. and Panksepp J., eds.), Marcel Dekker, New York.
Krieger D. T., Brownstein M. J., and Martin J. B. (1983) Brain Peptides John Wiley, New York.
Lenoir D., Battenberg E, Bloom F. E., and Milner R J. (1985) The rat brain specific gene 1B236 is expressed in cultures of fetal brain. Soc. Neurosci. Abst. 11, 1065.
Levi G., Banay-Schwartz M., and Ralteri M. (1978) Uptake, Exchange and Release of GABA in Isolated Nerve Endings, in Amino Acids as Chemical Transmitters (Fonnum F, ed.), Plenum, New York.
Marien M., Brien J., and Jhamandas K. (1983) Regional release of (3H) dopamine from rat brain in vitro: Effects of opioids on release induced by potassium, nicotine, and L-glutamic acid. Can. J. Physiol Pharmacol 61, 43–60.
McIlwain H and Rodnight R. (1962) Practical Neurochemistry Churchill, London.
McKelvy J. F., Charli J-L., Joseph-Bravo P., Sherman T., and Loudes C. (1980) Cellular Biochemistry of Brain Peptides: Biosynthesis, Degradation, Packaging, Transport and Release, in The Endocrine Functions of the Brain (Motta M., ed.) Raven, New York.
Myers R. D. (1972) Methods for perfusing different structures of the brain. Meth. Psychobiol 2, 169–211.
Nakanishi S., Inoue A, Kita T, Nakamura M., Chang A. C. Y., Cohen S. N, and Numa S. (1979) Nucleotide sequence of cloned cDNA for bovine corticotropin-β-lipotropin precursor. Nature 278, 423–427.
Nakata Y., Kusaka Y,, Yajima H., and Segawa T. (1981) Active uptake of substance P carboxy-terminal heptapeptide (5–11) into rat brain and rabbit spinal cord slices J. Neurochem 37, 1529–1534.
Neouillón A., Cheramy A, and Glowinski J. (1977) An adaptation of the push-pull cannula method to study the in vivo release of (3H)-dopamine synthesized from (3H)-tyrosine in cat caudate nucleus.Effect of various physical and pharmacological treatments. J. Neurochem. 28, 819–828.
Ohno N., Hashimoto K., Yunoki S., Takahara J., and Ofuji T. (1981) A perfusion method for examining arginine vasopressin release from hypothalamo-neurohypophyseal system. Acta Med. Okayama 35, 27–35.
Philippu A, Przuntek H., and Roensberg W. (1973) Superfusion of the hypothalamus with gamma-aminobutyric acid. Effect on the release of noradrenaline and blood pressure Naunyn Schmiedebergs Arch.Pharmacol 276, 103–118.
Pittman Q J, Hatton J D., and Bloom F E. (1981) Spontaneous activity in perfused hypothalamic slices: Dependence on calcium content of perfusate Exp Brain Res. 42, 49–52.
Pittman Q. J, Disturnal J., Riphagen C., Veale W L.,and Bauce L (1985) Perfusion Techniques for Neural Tissue, in Neuromethods vol. 1, General Techniques (Boulton A. A. and Baker G. B., eds.), Humana Press, Chfton, New Jersey.
Raiteri M., Angelini F, and Levi G. (1974) A simple apparatus for studying the release of neurotransmitters from synaptosomes Eur. J. Pharmacol. 25, 411–414.
Ramírez U. D. (1985) The Push-Pull Perfusion Technique in Neuroendocrinology, in In Vivo Perfusion and Release of Neuroactive Substances (Bayón A. and Drucker-Colín R., eds.), Academic, Orlando.
Redburn D A., Biela J., Shelton D, and Cotman C. W (1975) Stimulus-secretion coupling in vitro A rapid perfusion apparatus for monitoring efflux of transmitter substances from tissue samples. Anal. Biochem. 67, 268–278.
Robbins R. and Reichlin S. (1982) In Vitro Systems for the Study of Secretion and Synthesis of Hypothalamic Peptides, in Neuroendocrine Perspectives vol 1 (Miller E and MacLeod R, eds.), Elsevier, Amsterdam.
Segawa T, Nakata Y, Yajima H, and Kitagawa K (1977) Further observation of the lack of active uptake system for substance P in the central nervous system Japn. J Pharmacol 27, 573–580.
Sitges M., Possani L D, and Bayón A (1986) Noxiustoxin, a short chain toxin from the mexican scorpion Centruroides noxius induces transmitter release by blocking K+ permeability J Neurosci 6, 1570–1574.
Terry L. C. and Martin J, B. (1978) Hypothalamic hormones Subcellular distribution and mechanisms of release. Ann Rev. Pharmacol. Toxicol 18, 111–123.
Valverde C. and Bayón A (1983) Neuropeptides. Chemical Structure and Localization of the Producing Cells, in Aminoácidosy Péptidos en la Integracón de Funciones Nerviosas (Pasantes H and Arechiga H., eds ), UNAM, Mexico.
Velasco M, Velasco F, Pacheco M T., and Estrada-Villanueva F. (1985) Efficacy of the Push-Pull Perfusion Techniques in the Study of Sleep, Epilepsy and Tremor, in In Vivo Perfusion and Release of Neuroactive Substances (Bayón A. and Drucker-Colín R, eds.), Academic, Orlando.
Warberg J. (1982) Studies on the release mechanism for hypothalamic hormones Acta Endocrin. (suppl.) 250, 1–48.
Werman R (1966) Criteria for identification of a central nervous system transmitter Comp Biochem Physiol 18, 745–766.
Yaksh T. L. and Yamamura H I (1974) Factors affecting performance of the push-pull cannula in brain J. Appl. Physiol. 37, 428–434
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Bayón, A. (1987). In Vivo and In Vitro Studies on Peptide Release. In: Boulton, A.A., Baker, G.B., Pittman, Q.J. (eds) Peptides. Neuromethods, vol 6. Humana Press. https://doi.org/10.1385/0-89603-105-5:113
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DOI: https://doi.org/10.1385/0-89603-105-5:113
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